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Abstract
References
Discussion Forum (0)
1) Explain the persistence of multidrug resistance via plasmid carriage
2) Describe the bioinformatic challenges with mobile elements even when having a well-curated characterized isolates
3) Understand some bioinformatic strategies to better characterize multidrug resistant plasmid movement between stains and species
Sheppard AE, Stoesser N, German-Mesner I, Vegesana K, Sarah Walker A, Crook DW, Mathers AJ. TETyper: a bioinformatic pipeline for classifying variation and genetic contexts of transposable elements from short-read whole-genome sequencing data.Microb Genom. 2018 Nov 22. doi: 10.1099/mgen.0.000232. PMID: 30465646
Barry K, Wailan AM, Sheppard AE, Crook D, Vegesana K, Stoesser N, Parikh HI, Sebra R, Mathers AJ. Don't overlook the little guy: An evaluation of the frequency of small plasmids co-conjugating with larger carbapenemase gene containing plasmids. Plasmid. 2019 Mar 27. pii: S0147-619X(18)30155-0. doi: 10.1016/j.plasmid.2019.03.005. PMID:30928702
Sheppard AE, Stoesser N, Wilson DJ, Sebra R, Kasarskis A, Anson LW, Giess A, Pankhurst LJ, Vaughan A, Grim CJ, Cox HL, Yeh AJ; Modernising Medical Microbiology (MMM) Informatics Group, Sifri CD, Walker AS, Peto TE, Crook DW, Mathers AJ. Nested Russian Doll-like Genetic Mobility Drives Rapid Dissemination of the Carbapenem Resistance Gene blaKPC. Antimicrob Agents Chemother. (2016); 60(6):3767-78. PMID: 27067320.
35) Mathers AJ, Crook D, Vaughan A, Barry K, Vegesana K, Stoesser N, Parikh HI, Sebra R, Kotay S, Walker AS, Sheppard AE.Klebsiella quasipneumoniae provides a window into carbapenemase gene transfer, plasmid rearrangements and patient interactions with the hospital environment. Antimicrob Agents Chemother. 2019 Mar 25. pii: AAC.02513-18. doi: 10.1128/AAC.02513-18. PMID:30910889
There are still large knowledge gaps around horizontal gene transfer via plasmid exchange in real world settings. Using a well-curated longitudinal data set from a single hospital of a multispecies carbapenemase producing organisms where both the plumbing environment and hospitalized patients were involved and colonized has allowed a window into the complexity gene exchange. The presentation will cover some of the molecular epidemiologic/bioinformatic, environmental as well as biologic challenges with understanding and eliminating the dissemination of some of the most concerning genes of antibacterial resistance.
There are still large knowledge gaps around horizontal gene transfer via plasmid exchange in real world settings. Using a well-curated longitudinal data set from a single hospital of a multispecies carbapenemase producing organisms where both the plumbing environment and hospitalized patients were involved and colonized has allowed a window into the complexity gene exchange. The presentation will cover some of the molecular epidemiologic/bioinformatic, environmental as well as biologic challenges with understanding and eliminating the dissemination of some of the most concerning genes of antibacterial resistance.
Intergenus KPC carbapenemase transmission through promiscuous plasmids
Amy J. Mathers
Abstract
References
Discussion Forum (0)
1) Explain the persistence of multidrug resistance via plasmid carriage
2) Describe the bioinformatic challenges with mobile elements even when having a well-curated characterized isolates
3) Understand some bioinformatic strategies to better characterize multidrug resistant plasmid movement between stains and species
Sheppard AE, Stoesser N, German-Mesner I, Vegesana K, Sarah Walker A, Crook DW, Mathers AJ. TETyper: a bioinformatic pipeline for classifying variation and genetic contexts of transposable elements from short-read whole-genome sequencing data.Microb Genom. 2018 Nov 22. doi: 10.1099/mgen.0.000232. PMID: 30465646
Barry K, Wailan AM, Sheppard AE, Crook D, Vegesana K, Stoesser N, Parikh HI, Sebra R, Mathers AJ. Don't overlook the little guy: An evaluation of the frequency of small plasmids co-conjugating with larger carbapenemase gene containing plasmids. Plasmid. 2019 Mar 27. pii: S0147-619X(18)30155-0. doi: 10.1016/j.plasmid.2019.03.005. PMID:30928702
Sheppard AE, Stoesser N, Wilson DJ, Sebra R, Kasarskis A, Anson LW, Giess A, Pankhurst LJ, Vaughan A, Grim CJ, Cox HL, Yeh AJ; Modernising Medical Microbiology (MMM) Informatics Group, Sifri CD, Walker AS, Peto TE, Crook DW, Mathers AJ. Nested Russian Doll-like Genetic Mobility Drives Rapid Dissemination of the Carbapenem Resistance Gene blaKPC. Antimicrob Agents Chemother. (2016); 60(6):3767-78. PMID: 27067320.
35) Mathers AJ, Crook D, Vaughan A, Barry K, Vegesana K, Stoesser N, Parikh HI, Sebra R, Kotay S, Walker AS, Sheppard AE.Klebsiella quasipneumoniae provides a window into carbapenemase gene transfer, plasmid rearrangements and patient interactions with the hospital environment. Antimicrob Agents Chemother. 2019 Mar 25. pii: AAC.02513-18. doi: 10.1128/AAC.02513-18. PMID:30910889
There are still large knowledge gaps around horizontal gene transfer via plasmid exchange in real world settings. Using a well-curated longitudinal data set from a single hospital of a multispecies carbapenemase producing organisms where both the plumbing environment and hospitalized patients were involved and colonized has allowed a window into the complexity gene exchange. The presentation will cover some of the molecular epidemiologic/bioinformatic, environmental as well as biologic challenges with understanding and eliminating the dissemination of some of the most concerning genes of antibacterial resistance.
There are still large knowledge gaps around horizontal gene transfer via plasmid exchange in real world settings. Using a well-curated longitudinal data set from a single hospital of a multispecies carbapenemase producing organisms where both the plumbing environment and hospitalized patients were involved and colonized has allowed a window into the complexity gene exchange. The presentation will cover some of the molecular epidemiologic/bioinformatic, environmental as well as biologic challenges with understanding and eliminating the dissemination of some of the most concerning genes of antibacterial resistance.
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