Session Type: 1-hour Mini Oral Flash
Session Title: 1-hour Mini Oral Flash
Authors(s): E. Hernandez-Alonso (1), S. Barreault (1), K. Allouche (2), P. Jatteau (2), L. Augusto (1), P. Tissieres (1), F. Doucet-Populaire (1, 2), N. Bourgeois-Nicolaos (1, 2)
Authors Affiliations(s): (1) Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite Paris-Saclay, France, (2) AP-HP, Hôpital Antoine Béclère, Service de Microbiologie, Universite Paris-Saclay, France
Third Party Affiliation: SENSE study group
Background:
Enterobacter cloacae complex (ECC) has emerged as an important pathogen responsible for nosocomial infections such as neonatal bloodstream-infections. However, the clinical importance of the different members of ECC remains unclear as the species identification is unreliable especially by MALDI-TOF. We performed a sepsis historic cohort study, in neonatal intensive care units (NICU), to identify the ECC species precisely and characterize their antibiotic resistance.
Methods:88 significant strains of ECC isolated from premature-neonate blood cultures, from 2013 to 2019, were collected from 12 French hospitals. A method based on phylogenetic analysis by PCR-Sequencing was developed for species-level identification using the dnaJ gene. This method was validated by whole genome sequencing (WGS) on 20 strains. The antibiotic resistance profile was determined by EUCAST disk diffusion method.
Results:Six different species (E. hormaechei, E. bugandensis, E. cloacae, E. mori, E. ludwigii, E. cancerogenus) were identified with a high prevalence of E. hormaechei (52%, 46/88) followed by E. bugandensis (33%, 29/88). 22 strains were resistant to cefotaxime with different prevalence according the species: E. hormaechei (37%, 17/46) and E. bugandensis (10%, 3/29). Extended-spectrum β-lactamase phenotype was only detected for two E. bugandensis strains. All 88 strains were imipenem, meropenem, and amikacin sensible. Only 3 strains were resistant to ciprofloxacin. AmpC-type β-lactamase sequencing analysis showed that E. bugandensis strains carried the novel allele ACT-77/78/80/81 and 82. The global mortality rate was 37.14% (26/70) with 32% for E. hormaechei sepsis and 60% for E. bugandensis sepsis (p=0.02).
Conclusions:Using dnaJ gene phylogenetic analysis, we were able to assign precise species identification of ECC strains. These results confirm that E. bugandensis is an emerging species in NICUs and is associated with a poor prognosis in late-onset sepsis. This method based on PCR-sequencing can facilitate the management of nosocomial outbreaks and promotes rapid environmental monitoring. Finally, unlike other reported studies, E. bugandensis strains in this study were not resistant to antibiotics used in French NICUs.
Keyword(s): Neonatal sepsis, Enterobacter cloacae complex, Enterobacter bugandensisCOI Other: The authors have no conflicts of interest to declare
Session Type: 1-hour Mini Oral Flash
Session Title: 1-hour Mini Oral Flash
Authors(s): E. Hernandez-Alonso (1), S. Barreault (1), K. Allouche (2), P. Jatteau (2), L. Augusto (1), P. Tissieres (1), F. Doucet-Populaire (1, 2), N. Bourgeois-Nicolaos (1, 2)
Authors Affiliations(s): (1) Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite Paris-Saclay, France, (2) AP-HP, Hôpital Antoine Béclère, Service de Microbiologie, Universite Paris-Saclay, France
Third Party Affiliation: SENSE study group
Background:
Enterobacter cloacae complex (ECC) has emerged as an important pathogen responsible for nosocomial infections such as neonatal bloodstream-infections. However, the clinical importance of the different members of ECC remains unclear as the species identification is unreliable especially by MALDI-TOF. We performed a sepsis historic cohort study, in neonatal intensive care units (NICU), to identify the ECC species precisely and characterize their antibiotic resistance.
Methods:88 significant strains of ECC isolated from premature-neonate blood cultures, from 2013 to 2019, were collected from 12 French hospitals. A method based on phylogenetic analysis by PCR-Sequencing was developed for species-level identification using the dnaJ gene. This method was validated by whole genome sequencing (WGS) on 20 strains. The antibiotic resistance profile was determined by EUCAST disk diffusion method.
Results:Six different species (E. hormaechei, E. bugandensis, E. cloacae, E. mori, E. ludwigii, E. cancerogenus) were identified with a high prevalence of E. hormaechei (52%, 46/88) followed by E. bugandensis (33%, 29/88). 22 strains were resistant to cefotaxime with different prevalence according the species: E. hormaechei (37%, 17/46) and E. bugandensis (10%, 3/29). Extended-spectrum β-lactamase phenotype was only detected for two E. bugandensis strains. All 88 strains were imipenem, meropenem, and amikacin sensible. Only 3 strains were resistant to ciprofloxacin. AmpC-type β-lactamase sequencing analysis showed that E. bugandensis strains carried the novel allele ACT-77/78/80/81 and 82. The global mortality rate was 37.14% (26/70) with 32% for E. hormaechei sepsis and 60% for E. bugandensis sepsis (p=0.02).
Conclusions:Using dnaJ gene phylogenetic analysis, we were able to assign precise species identification of ECC strains. These results confirm that E. bugandensis is an emerging species in NICUs and is associated with a poor prognosis in late-onset sepsis. This method based on PCR-sequencing can facilitate the management of nosocomial outbreaks and promotes rapid environmental monitoring. Finally, unlike other reported studies, E. bugandensis strains in this study were not resistant to antibiotics used in French NICUs.
Keyword(s): Neonatal sepsis, Enterobacter cloacae complex, Enterobacter bugandensisCOI Other: The authors have no conflicts of interest to declare
Session Type: 1-hour Mini Oral Flash
Session Title: 1-hour Mini Oral Flash
Authors(s): E. Hernandez-Alonso (1), S. Barreault (1), K. Allouche (2), P. Jatteau (2), L. Augusto (1), P. Tissieres (1), F. Doucet-Populaire (1, 2), N. Bourgeois-Nicolaos (1, 2)
Authors Affiliations(s): (1) Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite Paris-Saclay, France, (2) AP-HP, Hôpital Antoine Béclère, Service de Microbiologie, Universite Paris-Saclay, France
Third Party Affiliation: SENSE study group
Background:
Enterobacter cloacae complex (ECC) has emerged as an important pathogen responsible for nosocomial infections such as neonatal bloodstream-infections. However, the clinical importance of the different members of ECC remains unclear as the species identification is unreliable especially by MALDI-TOF. We performed a sepsis historic cohort study, in neonatal intensive care units (NICU), to identify the ECC species precisely and characterize their antibiotic resistance.
Methods:88 significant strains of ECC isolated from premature-neonate blood cultures, from 2013 to 2019, were collected from 12 French hospitals. A method based on phylogenetic analysis by PCR-Sequencing was developed for species-level identification using the dnaJ gene. This method was validated by whole genome sequencing (WGS) on 20 strains. The antibiotic resistance profile was determined by EUCAST disk diffusion method.
Results:Six different species (E. hormaechei, E. bugandensis, E. cloacae, E. mori, E. ludwigii, E. cancerogenus) were identified with a high prevalence of E. hormaechei (52%, 46/88) followed by E. bugandensis (33%, 29/88). 22 strains were resistant to cefotaxime with different prevalence according the species: E. hormaechei (37%, 17/46) and E. bugandensis (10%, 3/29). Extended-spectrum β-lactamase phenotype was only detected for two E. bugandensis strains. All 88 strains were imipenem, meropenem, and amikacin sensible. Only 3 strains were resistant to ciprofloxacin. AmpC-type β-lactamase sequencing analysis showed that E. bugandensis strains carried the novel allele ACT-77/78/80/81 and 82. The global mortality rate was 37.14% (26/70) with 32% for E. hormaechei sepsis and 60% for E. bugandensis sepsis (p=0.02).
Conclusions:Using dnaJ gene phylogenetic analysis, we were able to assign precise species identification of ECC strains. These results confirm that E. bugandensis is an emerging species in NICUs and is associated with a poor prognosis in late-onset sepsis. This method based on PCR-sequencing can facilitate the management of nosocomial outbreaks and promotes rapid environmental monitoring. Finally, unlike other reported studies, E. bugandensis strains in this study were not resistant to antibiotics used in French NICUs.
Keyword(s): Neonatal sepsis, Enterobacter cloacae complex, Enterobacter bugandensisCOI Other: The authors have no conflicts of interest to declare
Session Type: 1-hour Mini Oral Flash
Session Title: 1-hour Mini Oral Flash
Authors(s): E. Hernandez-Alonso (1), S. Barreault (1), K. Allouche (2), P. Jatteau (2), L. Augusto (1), P. Tissieres (1), F. Doucet-Populaire (1, 2), N. Bourgeois-Nicolaos (1, 2)
Authors Affiliations(s): (1) Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universite Paris-Saclay, France, (2) AP-HP, Hôpital Antoine Béclère, Service de Microbiologie, Universite Paris-Saclay, France
Third Party Affiliation: SENSE study group
Background:
Enterobacter cloacae complex (ECC) has emerged as an important pathogen responsible for nosocomial infections such as neonatal bloodstream-infections. However, the clinical importance of the different members of ECC remains unclear as the species identification is unreliable especially by MALDI-TOF. We performed a sepsis historic cohort study, in neonatal intensive care units (NICU), to identify the ECC species precisely and characterize their antibiotic resistance.
Methods:88 significant strains of ECC isolated from premature-neonate blood cultures, from 2013 to 2019, were collected from 12 French hospitals. A method based on phylogenetic analysis by PCR-Sequencing was developed for species-level identification using the dnaJ gene. This method was validated by whole genome sequencing (WGS) on 20 strains. The antibiotic resistance profile was determined by EUCAST disk diffusion method.
Results:Six different species (E. hormaechei, E. bugandensis, E. cloacae, E. mori, E. ludwigii, E. cancerogenus) were identified with a high prevalence of E. hormaechei (52%, 46/88) followed by E. bugandensis (33%, 29/88). 22 strains were resistant to cefotaxime with different prevalence according the species: E. hormaechei (37%, 17/46) and E. bugandensis (10%, 3/29). Extended-spectrum β-lactamase phenotype was only detected for two E. bugandensis strains. All 88 strains were imipenem, meropenem, and amikacin sensible. Only 3 strains were resistant to ciprofloxacin. AmpC-type β-lactamase sequencing analysis showed that E. bugandensis strains carried the novel allele ACT-77/78/80/81 and 82. The global mortality rate was 37.14% (26/70) with 32% for E. hormaechei sepsis and 60% for E. bugandensis sepsis (p=0.02).
Conclusions:Using dnaJ gene phylogenetic analysis, we were able to assign precise species identification of ECC strains. These results confirm that E. bugandensis is an emerging species in NICUs and is associated with a poor prognosis in late-onset sepsis. This method based on PCR-sequencing can facilitate the management of nosocomial outbreaks and promotes rapid environmental monitoring. Finally, unlike other reported studies, E. bugandensis strains in this study were not resistant to antibiotics used in French NICUs.
Keyword(s): Neonatal sepsis, Enterobacter cloacae complex, Enterobacter bugandensisCOI Other: The authors have no conflicts of interest to declare