Session Type: 1,5-hour Oral Session
Session Title: 1,5-hour Oral Session
Authors(s): L. Lamberte (1), R. Kiu (2), R. Moran (1), W. Rowe (1), L. Hall (2, 3), W. Van Schaik (1)
Authors Affiliations(s): (1) University of Birmingham, United Kingdom, (2) The Quadram Institute, United Kingdom, (3) Technical University of Munich, Germany
Background:
Staphylococcus haemolyticus is a clinically important and highly common opportunistic pathogen. It is particularly important in preterm infants as it is one of the leading causes of late-onset sepsis (LOS). The success of S. haemolyticus in a clinical setting can be attributed to its involvement in the acquisition and dissemination of antimicrobial resistance. However, it is unclear whether horizontal gene transfer is also a contributor in the successful colonisation of S. haemolyticus in the gut microbiome of preterm infants.
Methods:To address this, we isolated S. haemolyticus from stool samples of a cohort of preterm infants. The stool samples used in this study were collected as part of the Wellcome Trust-funded multi-centre BAMBI (Baby Associated MicroBiota of the Intestine) study. We then used a combination of genomics and experimental approaches to study the horizontal transfer of resistance genes identified from our library of 57 S. haemolyticus isolates.
Results:Phylogenetic reconstruction categorised our isolates into three distinct groups, with one major group consisting 39/57 (68%) of the isolates from the cohort. Additionally, whole genome sequencing analysis detected the presence of a functional aminoglycoside resistance gene, aac(6’)-aph(2”), that is linked to Tn4001 and is widespread among the cohort.
Conclusions:Ultimately, results from these experiments show that antimicrobial resistance genes implicated in hospital-adapted S. haemolyticus isolates are also found within the gut microbiome of a cohort of preterm infants, and that S. haemolyticus is a reservoir for the spread of resistance genes.
COI Other: Funding by the Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom
Session Type: 1,5-hour Oral Session
Session Title: 1,5-hour Oral Session
Authors(s): L. Lamberte (1), R. Kiu (2), R. Moran (1), W. Rowe (1), L. Hall (2, 3), W. Van Schaik (1)
Authors Affiliations(s): (1) University of Birmingham, United Kingdom, (2) The Quadram Institute, United Kingdom, (3) Technical University of Munich, Germany
Background:
Staphylococcus haemolyticus is a clinically important and highly common opportunistic pathogen. It is particularly important in preterm infants as it is one of the leading causes of late-onset sepsis (LOS). The success of S. haemolyticus in a clinical setting can be attributed to its involvement in the acquisition and dissemination of antimicrobial resistance. However, it is unclear whether horizontal gene transfer is also a contributor in the successful colonisation of S. haemolyticus in the gut microbiome of preterm infants.
Methods:To address this, we isolated S. haemolyticus from stool samples of a cohort of preterm infants. The stool samples used in this study were collected as part of the Wellcome Trust-funded multi-centre BAMBI (Baby Associated MicroBiota of the Intestine) study. We then used a combination of genomics and experimental approaches to study the horizontal transfer of resistance genes identified from our library of 57 S. haemolyticus isolates.
Results:Phylogenetic reconstruction categorised our isolates into three distinct groups, with one major group consisting 39/57 (68%) of the isolates from the cohort. Additionally, whole genome sequencing analysis detected the presence of a functional aminoglycoside resistance gene, aac(6’)-aph(2”), that is linked to Tn4001 and is widespread among the cohort.
Conclusions:Ultimately, results from these experiments show that antimicrobial resistance genes implicated in hospital-adapted S. haemolyticus isolates are also found within the gut microbiome of a cohort of preterm infants, and that S. haemolyticus is a reservoir for the spread of resistance genes.
COI Other: Funding by the Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom
Session Type: 1,5-hour Oral Session
Session Title: 1,5-hour Oral Session
Authors(s): L. Lamberte (1), R. Kiu (2), R. Moran (1), W. Rowe (1), L. Hall (2, 3), W. Van Schaik (1)
Authors Affiliations(s): (1) University of Birmingham, United Kingdom, (2) The Quadram Institute, United Kingdom, (3) Technical University of Munich, Germany
Background:
Staphylococcus haemolyticus is a clinically important and highly common opportunistic pathogen. It is particularly important in preterm infants as it is one of the leading causes of late-onset sepsis (LOS). The success of S. haemolyticus in a clinical setting can be attributed to its involvement in the acquisition and dissemination of antimicrobial resistance. However, it is unclear whether horizontal gene transfer is also a contributor in the successful colonisation of S. haemolyticus in the gut microbiome of preterm infants.
Methods:To address this, we isolated S. haemolyticus from stool samples of a cohort of preterm infants. The stool samples used in this study were collected as part of the Wellcome Trust-funded multi-centre BAMBI (Baby Associated MicroBiota of the Intestine) study. We then used a combination of genomics and experimental approaches to study the horizontal transfer of resistance genes identified from our library of 57 S. haemolyticus isolates.
Results:Phylogenetic reconstruction categorised our isolates into three distinct groups, with one major group consisting 39/57 (68%) of the isolates from the cohort. Additionally, whole genome sequencing analysis detected the presence of a functional aminoglycoside resistance gene, aac(6’)-aph(2”), that is linked to Tn4001 and is widespread among the cohort.
Conclusions:Ultimately, results from these experiments show that antimicrobial resistance genes implicated in hospital-adapted S. haemolyticus isolates are also found within the gut microbiome of a cohort of preterm infants, and that S. haemolyticus is a reservoir for the spread of resistance genes.
COI Other: Funding by the Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom
Session Type: 1,5-hour Oral Session
Session Title: 1,5-hour Oral Session
Authors(s): L. Lamberte (1), R. Kiu (2), R. Moran (1), W. Rowe (1), L. Hall (2, 3), W. Van Schaik (1)
Authors Affiliations(s): (1) University of Birmingham, United Kingdom, (2) The Quadram Institute, United Kingdom, (3) Technical University of Munich, Germany
Background:
Staphylococcus haemolyticus is a clinically important and highly common opportunistic pathogen. It is particularly important in preterm infants as it is one of the leading causes of late-onset sepsis (LOS). The success of S. haemolyticus in a clinical setting can be attributed to its involvement in the acquisition and dissemination of antimicrobial resistance. However, it is unclear whether horizontal gene transfer is also a contributor in the successful colonisation of S. haemolyticus in the gut microbiome of preterm infants.
Methods:To address this, we isolated S. haemolyticus from stool samples of a cohort of preterm infants. The stool samples used in this study were collected as part of the Wellcome Trust-funded multi-centre BAMBI (Baby Associated MicroBiota of the Intestine) study. We then used a combination of genomics and experimental approaches to study the horizontal transfer of resistance genes identified from our library of 57 S. haemolyticus isolates.
Results:Phylogenetic reconstruction categorised our isolates into three distinct groups, with one major group consisting 39/57 (68%) of the isolates from the cohort. Additionally, whole genome sequencing analysis detected the presence of a functional aminoglycoside resistance gene, aac(6’)-aph(2”), that is linked to Tn4001 and is widespread among the cohort.
Conclusions:Ultimately, results from these experiments show that antimicrobial resistance genes implicated in hospital-adapted S. haemolyticus isolates are also found within the gut microbiome of a cohort of preterm infants, and that S. haemolyticus is a reservoir for the spread of resistance genes.
COI Other: Funding by the Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom