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Abstract
Discussion Forum (0)
Abstract number: 1088

Session Type: ePosters

Session Title: ePosters

Authors(s): N.A. Saif (1), J. Cobo-Díaz (2), M. Elserafy (1), I. Elsheikh (1), A. Alvarez-Ordóñez (2), S. Mouftah (1), M. Elhadidy (1)

Authors Affiliations(s): (1) Zewail City, Egypt, (2) Universidad de León, Egypt

Background:

Campylobacter jejuni (C. jejuni), is considered among the most common bacterial causes of human bacterial gastroenteritis  worldwide. The epidemiology and transmission dynamics of campylobacteriosis in Egypt remain poorly defined due to the limited use  of high-resolution typing methods.

Methods:

In this pilot study, we evaluated the discriminatory power of multiple typing ‘gene-by-gene based’  techniques to characterize C. jejuni obtained from different sources and estimate the relative contribution of different potential sources  of C. jejuni infection in Egypt. Whole genome sequencing (WGS) was performed on 90 C. jejuni isolates recovered from clinical  samples, retail chicken, and dairy products in Egypt in 2017-2018. Comparative genomic analyses were performed using conventional  seven-locus multilocus sequence typing (MLST), ribosomal multilocus sequence typing (rMLST), core genome multilocus sequence  typing (cgMLST), and comparative genomic fingerprinting (CGF40). The probabilistic source attribution was performed via  STRUCTURE software using MLST, CGF40, cgMLST, and allelic variation in 15 host-segregating (HS) markers.

Results:

Comparison of the discriminatory power of the aforementioned genotyping methods using the Simpson diversity index revealed cgMLST to be the most  discriminatory method, followed by HS markers. The source attribution analysis showed the role of retail chicken as a source of infection  among clinical cases in Egypt, when HS and cgMLST were used (64.2% and 52.3% of clinical isolates were assigned to this source,  respectively). Interestingly, the cattle reservoir was also identified as a contributor to C. jejuni infection in Egypt; 35.8% and 47.7% of 36 clinical isolates were assigned to this source by HS and cgMLST, respectively. Here, we provided an evidence of the importance of 37 using high resolution WGS typing methods to facilitate source tracking of C. jejuni.

Conclusions:

Our findings suggest the importance of non-poultry  sources, together with the previously reported role of retail chicken in human campylobacteriosisis in Egypt that can provide insights to  inform national control measures.

Keyword(s): Campylobacter jejuni, Host-associated genetic signatures, Source attribution

COI Institutional Grants: Yes
COI Other: Zewail City internal research fund (agreement number ZC 004-2019) and joint ASRT/BA research grant (Project number 1110)
Abstract number: 1088

Session Type: ePosters

Session Title: ePosters

Authors(s): N.A. Saif (1), J. Cobo-Díaz (2), M. Elserafy (1), I. Elsheikh (1), A. Alvarez-Ordóñez (2), S. Mouftah (1), M. Elhadidy (1)

Authors Affiliations(s): (1) Zewail City, Egypt, (2) Universidad de León, Egypt

Background:

Campylobacter jejuni (C. jejuni), is considered among the most common bacterial causes of human bacterial gastroenteritis  worldwide. The epidemiology and transmission dynamics of campylobacteriosis in Egypt remain poorly defined due to the limited use  of high-resolution typing methods.

Methods:

In this pilot study, we evaluated the discriminatory power of multiple typing ‘gene-by-gene based’  techniques to characterize C. jejuni obtained from different sources and estimate the relative contribution of different potential sources  of C. jejuni infection in Egypt. Whole genome sequencing (WGS) was performed on 90 C. jejuni isolates recovered from clinical  samples, retail chicken, and dairy products in Egypt in 2017-2018. Comparative genomic analyses were performed using conventional  seven-locus multilocus sequence typing (MLST), ribosomal multilocus sequence typing (rMLST), core genome multilocus sequence  typing (cgMLST), and comparative genomic fingerprinting (CGF40). The probabilistic source attribution was performed via  STRUCTURE software using MLST, CGF40, cgMLST, and allelic variation in 15 host-segregating (HS) markers.

Results:

Comparison of the discriminatory power of the aforementioned genotyping methods using the Simpson diversity index revealed cgMLST to be the most  discriminatory method, followed by HS markers. The source attribution analysis showed the role of retail chicken as a source of infection  among clinical cases in Egypt, when HS and cgMLST were used (64.2% and 52.3% of clinical isolates were assigned to this source,  respectively). Interestingly, the cattle reservoir was also identified as a contributor to C. jejuni infection in Egypt; 35.8% and 47.7% of 36 clinical isolates were assigned to this source by HS and cgMLST, respectively. Here, we provided an evidence of the importance of 37 using high resolution WGS typing methods to facilitate source tracking of C. jejuni.

Conclusions:

Our findings suggest the importance of non-poultry  sources, together with the previously reported role of retail chicken in human campylobacteriosisis in Egypt that can provide insights to  inform national control measures.

Keyword(s): Campylobacter jejuni, Host-associated genetic signatures, Source attribution

COI Institutional Grants: Yes
COI Other: Zewail City internal research fund (agreement number ZC 004-2019) and joint ASRT/BA research grant (Project number 1110)
A pilot study revealing host-associated genetic signatures for source attribution and stress resistance of sporadic Campylobacter jejuni infection in Egypt
Mrs. Nehal Aasem Saif
Mrs. Nehal Aasem Saif
ESCMID eAcademy. Saif N. 07/09/2021; 330157; 1088
user
Mrs. Nehal Aasem Saif
Abstract
Discussion Forum (0)
Abstract number: 1088

Session Type: ePosters

Session Title: ePosters

Authors(s): N.A. Saif (1), J. Cobo-Díaz (2), M. Elserafy (1), I. Elsheikh (1), A. Alvarez-Ordóñez (2), S. Mouftah (1), M. Elhadidy (1)

Authors Affiliations(s): (1) Zewail City, Egypt, (2) Universidad de León, Egypt

Background:

Campylobacter jejuni (C. jejuni), is considered among the most common bacterial causes of human bacterial gastroenteritis  worldwide. The epidemiology and transmission dynamics of campylobacteriosis in Egypt remain poorly defined due to the limited use  of high-resolution typing methods.

Methods:

In this pilot study, we evaluated the discriminatory power of multiple typing ‘gene-by-gene based’  techniques to characterize C. jejuni obtained from different sources and estimate the relative contribution of different potential sources  of C. jejuni infection in Egypt. Whole genome sequencing (WGS) was performed on 90 C. jejuni isolates recovered from clinical  samples, retail chicken, and dairy products in Egypt in 2017-2018. Comparative genomic analyses were performed using conventional  seven-locus multilocus sequence typing (MLST), ribosomal multilocus sequence typing (rMLST), core genome multilocus sequence  typing (cgMLST), and comparative genomic fingerprinting (CGF40). The probabilistic source attribution was performed via  STRUCTURE software using MLST, CGF40, cgMLST, and allelic variation in 15 host-segregating (HS) markers.

Results:

Comparison of the discriminatory power of the aforementioned genotyping methods using the Simpson diversity index revealed cgMLST to be the most  discriminatory method, followed by HS markers. The source attribution analysis showed the role of retail chicken as a source of infection  among clinical cases in Egypt, when HS and cgMLST were used (64.2% and 52.3% of clinical isolates were assigned to this source,  respectively). Interestingly, the cattle reservoir was also identified as a contributor to C. jejuni infection in Egypt; 35.8% and 47.7% of 36 clinical isolates were assigned to this source by HS and cgMLST, respectively. Here, we provided an evidence of the importance of 37 using high resolution WGS typing methods to facilitate source tracking of C. jejuni.

Conclusions:

Our findings suggest the importance of non-poultry  sources, together with the previously reported role of retail chicken in human campylobacteriosisis in Egypt that can provide insights to  inform national control measures.

Keyword(s): Campylobacter jejuni, Host-associated genetic signatures, Source attribution

COI Institutional Grants: Yes
COI Other: Zewail City internal research fund (agreement number ZC 004-2019) and joint ASRT/BA research grant (Project number 1110)
Abstract number: 1088

Session Type: ePosters

Session Title: ePosters

Authors(s): N.A. Saif (1), J. Cobo-Díaz (2), M. Elserafy (1), I. Elsheikh (1), A. Alvarez-Ordóñez (2), S. Mouftah (1), M. Elhadidy (1)

Authors Affiliations(s): (1) Zewail City, Egypt, (2) Universidad de León, Egypt

Background:

Campylobacter jejuni (C. jejuni), is considered among the most common bacterial causes of human bacterial gastroenteritis  worldwide. The epidemiology and transmission dynamics of campylobacteriosis in Egypt remain poorly defined due to the limited use  of high-resolution typing methods.

Methods:

In this pilot study, we evaluated the discriminatory power of multiple typing ‘gene-by-gene based’  techniques to characterize C. jejuni obtained from different sources and estimate the relative contribution of different potential sources  of C. jejuni infection in Egypt. Whole genome sequencing (WGS) was performed on 90 C. jejuni isolates recovered from clinical  samples, retail chicken, and dairy products in Egypt in 2017-2018. Comparative genomic analyses were performed using conventional  seven-locus multilocus sequence typing (MLST), ribosomal multilocus sequence typing (rMLST), core genome multilocus sequence  typing (cgMLST), and comparative genomic fingerprinting (CGF40). The probabilistic source attribution was performed via  STRUCTURE software using MLST, CGF40, cgMLST, and allelic variation in 15 host-segregating (HS) markers.

Results:

Comparison of the discriminatory power of the aforementioned genotyping methods using the Simpson diversity index revealed cgMLST to be the most  discriminatory method, followed by HS markers. The source attribution analysis showed the role of retail chicken as a source of infection  among clinical cases in Egypt, when HS and cgMLST were used (64.2% and 52.3% of clinical isolates were assigned to this source,  respectively). Interestingly, the cattle reservoir was also identified as a contributor to C. jejuni infection in Egypt; 35.8% and 47.7% of 36 clinical isolates were assigned to this source by HS and cgMLST, respectively. Here, we provided an evidence of the importance of 37 using high resolution WGS typing methods to facilitate source tracking of C. jejuni.

Conclusions:

Our findings suggest the importance of non-poultry  sources, together with the previously reported role of retail chicken in human campylobacteriosisis in Egypt that can provide insights to  inform national control measures.

Keyword(s): Campylobacter jejuni, Host-associated genetic signatures, Source attribution

COI Institutional Grants: Yes
COI Other: Zewail City internal research fund (agreement number ZC 004-2019) and joint ASRT/BA research grant (Project number 1110)

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