Session Type: ePosters
Session Title: ePosters
Authors(s): N.A. Saif (1), J. Cobo-Díaz (2), M. Elserafy (1), I. Elsheikh (1), A. Alvarez-Ordóñez (2), S. Mouftah (1), M. Elhadidy (1)
Authors Affiliations(s): (1) Zewail City, Egypt, (2) Universidad de León, Egypt
Background:
Campylobacter jejuni (C. jejuni), is considered among the most common bacterial causes of human bacterial gastroenteritis worldwide. The epidemiology and transmission dynamics of campylobacteriosis in Egypt remain poorly defined due to the limited use of high-resolution typing methods.
Methods:In this pilot study, we evaluated the discriminatory power of multiple typing ‘gene-by-gene based’ techniques to characterize C. jejuni obtained from different sources and estimate the relative contribution of different potential sources of C. jejuni infection in Egypt. Whole genome sequencing (WGS) was performed on 90 C. jejuni isolates recovered from clinical samples, retail chicken, and dairy products in Egypt in 2017-2018. Comparative genomic analyses were performed using conventional seven-locus multilocus sequence typing (MLST), ribosomal multilocus sequence typing (rMLST), core genome multilocus sequence typing (cgMLST), and comparative genomic fingerprinting (CGF40). The probabilistic source attribution was performed via STRUCTURE software using MLST, CGF40, cgMLST, and allelic variation in 15 host-segregating (HS) markers.
Results:Comparison of the discriminatory power of the aforementioned genotyping methods using the Simpson diversity index revealed cgMLST to be the most discriminatory method, followed by HS markers. The source attribution analysis showed the role of retail chicken as a source of infection among clinical cases in Egypt, when HS and cgMLST were used (64.2% and 52.3% of clinical isolates were assigned to this source, respectively). Interestingly, the cattle reservoir was also identified as a contributor to C. jejuni infection in Egypt; 35.8% and 47.7% of 36 clinical isolates were assigned to this source by HS and cgMLST, respectively. Here, we provided an evidence of the importance of 37 using high resolution WGS typing methods to facilitate source tracking of C. jejuni.
Conclusions:Our findings suggest the importance of non-poultry sources, together with the previously reported role of retail chicken in human campylobacteriosisis in Egypt that can provide insights to inform national control measures.
Keyword(s): Campylobacter jejuni, Host-associated genetic signatures, Source attributionCOI Institutional Grants: Yes
COI Other: Zewail City internal research fund (agreement number ZC 004-2019) and joint ASRT/BA research grant (Project number 1110)
Session Type: ePosters
Session Title: ePosters
Authors(s): N.A. Saif (1), J. Cobo-Díaz (2), M. Elserafy (1), I. Elsheikh (1), A. Alvarez-Ordóñez (2), S. Mouftah (1), M. Elhadidy (1)
Authors Affiliations(s): (1) Zewail City, Egypt, (2) Universidad de León, Egypt
Background:
Campylobacter jejuni (C. jejuni), is considered among the most common bacterial causes of human bacterial gastroenteritis worldwide. The epidemiology and transmission dynamics of campylobacteriosis in Egypt remain poorly defined due to the limited use of high-resolution typing methods.
Methods:In this pilot study, we evaluated the discriminatory power of multiple typing ‘gene-by-gene based’ techniques to characterize C. jejuni obtained from different sources and estimate the relative contribution of different potential sources of C. jejuni infection in Egypt. Whole genome sequencing (WGS) was performed on 90 C. jejuni isolates recovered from clinical samples, retail chicken, and dairy products in Egypt in 2017-2018. Comparative genomic analyses were performed using conventional seven-locus multilocus sequence typing (MLST), ribosomal multilocus sequence typing (rMLST), core genome multilocus sequence typing (cgMLST), and comparative genomic fingerprinting (CGF40). The probabilistic source attribution was performed via STRUCTURE software using MLST, CGF40, cgMLST, and allelic variation in 15 host-segregating (HS) markers.
Results:Comparison of the discriminatory power of the aforementioned genotyping methods using the Simpson diversity index revealed cgMLST to be the most discriminatory method, followed by HS markers. The source attribution analysis showed the role of retail chicken as a source of infection among clinical cases in Egypt, when HS and cgMLST were used (64.2% and 52.3% of clinical isolates were assigned to this source, respectively). Interestingly, the cattle reservoir was also identified as a contributor to C. jejuni infection in Egypt; 35.8% and 47.7% of 36 clinical isolates were assigned to this source by HS and cgMLST, respectively. Here, we provided an evidence of the importance of 37 using high resolution WGS typing methods to facilitate source tracking of C. jejuni.
Conclusions:Our findings suggest the importance of non-poultry sources, together with the previously reported role of retail chicken in human campylobacteriosisis in Egypt that can provide insights to inform national control measures.
Keyword(s): Campylobacter jejuni, Host-associated genetic signatures, Source attributionCOI Institutional Grants: Yes
COI Other: Zewail City internal research fund (agreement number ZC 004-2019) and joint ASRT/BA research grant (Project number 1110)