Session Type: ePosters
Session Title: ePosters
Authors(s): A. Bouyssi (1), M. Delles (1), T. Déméautis (1), F. Persat (2), G. Devouassoux (2), A. Bentaher (3), J. Menotti (2)
Authors Affiliations(s): (1) Université Claude Bernard Lyon 1, France, (2) Université Claude Bernard Lyon 1/ Hospices Civils de Lyon, France, (3) Université Claude Bernard Lyon 1/ INSERM, France
Background:
Chronic obstructive pulmonary disease (COPD) is characterised by a chronic inflammation of respiratory tract, leading to irreversible lung injury and emphysema, responsible for shortness of breath and exacerbations, frequently elicited by infections. Regarding this latter, airway exposure to Aspergillus fumigatus spores may be responsible for episodic exacerbations in COPD patients. The aim of the study was to investigate the inflammatory response involved in A. fumigatus spores-mediated COPD exacerbation using a clinically relevant cell culture model.
Methods:To better understand these inflammatory processes, we developed a co-culture model of murine macrophages and alveolar epithelial cells exposed to cigarette smoke extract and/or viable spores of A. fumigatus. The presence of the two cell types in co-culture was confirmed using immunofluorescent microscopy. Expression of genes of interest was quantified using TaqMan™ gene expression assays. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparisons test.
Results:In cells treated by spores alone versus untreated cells, we observed induction of gene expression for IL1β (p = 0.0013), CXCL-1 (p = 0.0119), MIP-2 (p < 0.0001), TNF-α (p = 0.0062) and GM-CSF (p = 0.0066). In cells treated with CSE alone, an increase of gene expression for MIP-2 (p = 0.0122) was observed, while IL1β (p = 0.0015) and GM-CSF (p = 0.0473) gene expressions were decreased. In cells treated with CSE and spores versus CSE alone, MIP-2 gene expression was increased (p = 0.0003).
Conclusions:
Both macrophages and alveolar epithelial cells seem to respond differently to our treatments: individual or combined. Interestingly, our preliminary data on MIP-2 induction suggest the importance of this cytokine in the exacerbation phase that is usually characterised by massive infiltration of neutrophils. The relevance of this finding and others to disease development and A. fumigatus-mediated exacerbation phase is ongoing. Last but not least, this cell model could be useful to study COPD pathogenesis both in stable and exacerbation states.
Keyword(s): Aspergillus fumigatus, COPD, Inflammatory responseCOI Other: This study has received a support grant from FINOVI (Fondation Innovations en Infectiologie). A. Bouyssi has received a support contract for her PhD from Agir Pour les Maladies Chroniques and an Award from the Société Française de Mycologie Médicale.
Session Type: ePosters
Session Title: ePosters
Authors(s): A. Bouyssi (1), M. Delles (1), T. Déméautis (1), F. Persat (2), G. Devouassoux (2), A. Bentaher (3), J. Menotti (2)
Authors Affiliations(s): (1) Université Claude Bernard Lyon 1, France, (2) Université Claude Bernard Lyon 1/ Hospices Civils de Lyon, France, (3) Université Claude Bernard Lyon 1/ INSERM, France
Background:
Chronic obstructive pulmonary disease (COPD) is characterised by a chronic inflammation of respiratory tract, leading to irreversible lung injury and emphysema, responsible for shortness of breath and exacerbations, frequently elicited by infections. Regarding this latter, airway exposure to Aspergillus fumigatus spores may be responsible for episodic exacerbations in COPD patients. The aim of the study was to investigate the inflammatory response involved in A. fumigatus spores-mediated COPD exacerbation using a clinically relevant cell culture model.
Methods:To better understand these inflammatory processes, we developed a co-culture model of murine macrophages and alveolar epithelial cells exposed to cigarette smoke extract and/or viable spores of A. fumigatus. The presence of the two cell types in co-culture was confirmed using immunofluorescent microscopy. Expression of genes of interest was quantified using TaqMan™ gene expression assays. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparisons test.
Results:In cells treated by spores alone versus untreated cells, we observed induction of gene expression for IL1β (p = 0.0013), CXCL-1 (p = 0.0119), MIP-2 (p < 0.0001), TNF-α (p = 0.0062) and GM-CSF (p = 0.0066). In cells treated with CSE alone, an increase of gene expression for MIP-2 (p = 0.0122) was observed, while IL1β (p = 0.0015) and GM-CSF (p = 0.0473) gene expressions were decreased. In cells treated with CSE and spores versus CSE alone, MIP-2 gene expression was increased (p = 0.0003).
Conclusions:
Both macrophages and alveolar epithelial cells seem to respond differently to our treatments: individual or combined. Interestingly, our preliminary data on MIP-2 induction suggest the importance of this cytokine in the exacerbation phase that is usually characterised by massive infiltration of neutrophils. The relevance of this finding and others to disease development and A. fumigatus-mediated exacerbation phase is ongoing. Last but not least, this cell model could be useful to study COPD pathogenesis both in stable and exacerbation states.
Keyword(s): Aspergillus fumigatus, COPD, Inflammatory responseCOI Other: This study has received a support grant from FINOVI (Fondation Innovations en Infectiologie). A. Bouyssi has received a support contract for her PhD from Agir Pour les Maladies Chroniques and an Award from the Société Française de Mycologie Médicale.
Session Type: ePosters
Session Title: ePosters
Authors(s): A. Bouyssi (1), M. Delles (1), T. Déméautis (1), F. Persat (2), G. Devouassoux (2), A. Bentaher (3), J. Menotti (2)
Authors Affiliations(s): (1) Université Claude Bernard Lyon 1, France, (2) Université Claude Bernard Lyon 1/ Hospices Civils de Lyon, France, (3) Université Claude Bernard Lyon 1/ INSERM, France
Background:
Chronic obstructive pulmonary disease (COPD) is characterised by a chronic inflammation of respiratory tract, leading to irreversible lung injury and emphysema, responsible for shortness of breath and exacerbations, frequently elicited by infections. Regarding this latter, airway exposure to Aspergillus fumigatus spores may be responsible for episodic exacerbations in COPD patients. The aim of the study was to investigate the inflammatory response involved in A. fumigatus spores-mediated COPD exacerbation using a clinically relevant cell culture model.
Methods:To better understand these inflammatory processes, we developed a co-culture model of murine macrophages and alveolar epithelial cells exposed to cigarette smoke extract and/or viable spores of A. fumigatus. The presence of the two cell types in co-culture was confirmed using immunofluorescent microscopy. Expression of genes of interest was quantified using TaqMan™ gene expression assays. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparisons test.
Results:In cells treated by spores alone versus untreated cells, we observed induction of gene expression for IL1β (p = 0.0013), CXCL-1 (p = 0.0119), MIP-2 (p < 0.0001), TNF-α (p = 0.0062) and GM-CSF (p = 0.0066). In cells treated with CSE alone, an increase of gene expression for MIP-2 (p = 0.0122) was observed, while IL1β (p = 0.0015) and GM-CSF (p = 0.0473) gene expressions were decreased. In cells treated with CSE and spores versus CSE alone, MIP-2 gene expression was increased (p = 0.0003).
Conclusions:
Both macrophages and alveolar epithelial cells seem to respond differently to our treatments: individual or combined. Interestingly, our preliminary data on MIP-2 induction suggest the importance of this cytokine in the exacerbation phase that is usually characterised by massive infiltration of neutrophils. The relevance of this finding and others to disease development and A. fumigatus-mediated exacerbation phase is ongoing. Last but not least, this cell model could be useful to study COPD pathogenesis both in stable and exacerbation states.
Keyword(s): Aspergillus fumigatus, COPD, Inflammatory responseCOI Other: This study has received a support grant from FINOVI (Fondation Innovations en Infectiologie). A. Bouyssi has received a support contract for her PhD from Agir Pour les Maladies Chroniques and an Award from the Société Française de Mycologie Médicale.
Session Type: ePosters
Session Title: ePosters
Authors(s): A. Bouyssi (1), M. Delles (1), T. Déméautis (1), F. Persat (2), G. Devouassoux (2), A. Bentaher (3), J. Menotti (2)
Authors Affiliations(s): (1) Université Claude Bernard Lyon 1, France, (2) Université Claude Bernard Lyon 1/ Hospices Civils de Lyon, France, (3) Université Claude Bernard Lyon 1/ INSERM, France
Background:
Chronic obstructive pulmonary disease (COPD) is characterised by a chronic inflammation of respiratory tract, leading to irreversible lung injury and emphysema, responsible for shortness of breath and exacerbations, frequently elicited by infections. Regarding this latter, airway exposure to Aspergillus fumigatus spores may be responsible for episodic exacerbations in COPD patients. The aim of the study was to investigate the inflammatory response involved in A. fumigatus spores-mediated COPD exacerbation using a clinically relevant cell culture model.
Methods:To better understand these inflammatory processes, we developed a co-culture model of murine macrophages and alveolar epithelial cells exposed to cigarette smoke extract and/or viable spores of A. fumigatus. The presence of the two cell types in co-culture was confirmed using immunofluorescent microscopy. Expression of genes of interest was quantified using TaqMan™ gene expression assays. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparisons test.
Results:In cells treated by spores alone versus untreated cells, we observed induction of gene expression for IL1β (p = 0.0013), CXCL-1 (p = 0.0119), MIP-2 (p < 0.0001), TNF-α (p = 0.0062) and GM-CSF (p = 0.0066). In cells treated with CSE alone, an increase of gene expression for MIP-2 (p = 0.0122) was observed, while IL1β (p = 0.0015) and GM-CSF (p = 0.0473) gene expressions were decreased. In cells treated with CSE and spores versus CSE alone, MIP-2 gene expression was increased (p = 0.0003).
Conclusions:
Both macrophages and alveolar epithelial cells seem to respond differently to our treatments: individual or combined. Interestingly, our preliminary data on MIP-2 induction suggest the importance of this cytokine in the exacerbation phase that is usually characterised by massive infiltration of neutrophils. The relevance of this finding and others to disease development and A. fumigatus-mediated exacerbation phase is ongoing. Last but not least, this cell model could be useful to study COPD pathogenesis both in stable and exacerbation states.
Keyword(s): Aspergillus fumigatus, COPD, Inflammatory responseCOI Other: This study has received a support grant from FINOVI (Fondation Innovations en Infectiologie). A. Bouyssi has received a support contract for her PhD from Agir Pour les Maladies Chroniques and an Award from the Société Française de Mycologie Médicale.