Session Type: 1-hour ePoster Review
Session Title: 1-hour ePoster Review
Authors(s): X.F. Chen, M. Xiao, X. Hou, Y.C. Xu
Authors Affiliations(s): Peking Union Medical College Hospital, China
Background:
The emergence of a multidrug-resistant yeast, Candida auris, has drawn attention to the closely related species from the Candida haemulonii complex includeing Candida haemulonii, Candida duobushaemulonii, Candida pseudohaemulonii, and Candida haemulonii var. vulnera. The goal of this study was to investigate the genetic relationships and drug-resistance profiles among isolates of the C. duobushaemulonii from different cities and to examine possible evidence of transmission if it existed.
Methods:The12 Candida duobushaemulonii isolates causing invasive fungal diseases were collected from nine tertiary hospitals through CHIF-NET. We used antifungal susceptibility testing and whole-genome sequencing (WGS) to investigate drug resistance and genetic diversity among isolates of Candida duobushaemulonii from different geographic areas of China.
Results:Between 2010 and 2017, 12 isolates of Candida duobushaemulonii from China; In the China, 41.7% of Candida duobushaemulonii was isolated from blood, and other invasive sites (Table 1). Phylogenetic analysis using SNPs called against the Candida duobushaemulonii reference strain B09383. Genetic relationships among Candida duobushaemulonii isolates are shown in (Figure 1). The average pairwise difference between the isolates was 691 SNPs (range 15–1273), and there was distinct phylogeographic population structure. Three isolates, F4444, F4464 were different from each other by fewer than 15 SNPs. These two isolates were recovered from the same patients. In addition, two isolates, F4490, F4560, differed by fewer than 90 SNPs recovered from difference hospital. We observed high levels of susceptibility to amphotericin B among 12 tested Candida duobushaemulonii isolates: all had elevated MICs from 4μg/mL to >8μg/ ml. All isolates had elevated MICs of fluconazole ranging from 64 to 256μg/ml (Table 2). Interesting, these isolates don’t have the known mutation in ERG11, only with synonymous mutation.
Conclusions:Our results indicate that, although we are not observing the widespread of the Candida duobushaemulonii, at least these isolates had elevated MICs of fluconazole. We need to investigated the antifungal-resistance mechanism.
Keyword(s): Candida duobushaemulonii, multidrug-resistant yeast, whole-genome sequencingSession Type: 1-hour ePoster Review
Session Title: 1-hour ePoster Review
Authors(s): X.F. Chen, M. Xiao, X. Hou, Y.C. Xu
Authors Affiliations(s): Peking Union Medical College Hospital, China
Background:
The emergence of a multidrug-resistant yeast, Candida auris, has drawn attention to the closely related species from the Candida haemulonii complex includeing Candida haemulonii, Candida duobushaemulonii, Candida pseudohaemulonii, and Candida haemulonii var. vulnera. The goal of this study was to investigate the genetic relationships and drug-resistance profiles among isolates of the C. duobushaemulonii from different cities and to examine possible evidence of transmission if it existed.
Methods:The12 Candida duobushaemulonii isolates causing invasive fungal diseases were collected from nine tertiary hospitals through CHIF-NET. We used antifungal susceptibility testing and whole-genome sequencing (WGS) to investigate drug resistance and genetic diversity among isolates of Candida duobushaemulonii from different geographic areas of China.
Results:Between 2010 and 2017, 12 isolates of Candida duobushaemulonii from China; In the China, 41.7% of Candida duobushaemulonii was isolated from blood, and other invasive sites (Table 1). Phylogenetic analysis using SNPs called against the Candida duobushaemulonii reference strain B09383. Genetic relationships among Candida duobushaemulonii isolates are shown in (Figure 1). The average pairwise difference between the isolates was 691 SNPs (range 15–1273), and there was distinct phylogeographic population structure. Three isolates, F4444, F4464 were different from each other by fewer than 15 SNPs. These two isolates were recovered from the same patients. In addition, two isolates, F4490, F4560, differed by fewer than 90 SNPs recovered from difference hospital. We observed high levels of susceptibility to amphotericin B among 12 tested Candida duobushaemulonii isolates: all had elevated MICs from 4μg/mL to >8μg/ ml. All isolates had elevated MICs of fluconazole ranging from 64 to 256μg/ml (Table 2). Interesting, these isolates don’t have the known mutation in ERG11, only with synonymous mutation.
Conclusions:Our results indicate that, although we are not observing the widespread of the Candida duobushaemulonii, at least these isolates had elevated MICs of fluconazole. We need to investigated the antifungal-resistance mechanism.
Keyword(s): Candida duobushaemulonii, multidrug-resistant yeast, whole-genome sequencing
Session Type: 1-hour ePoster Review
Session Title: 1-hour ePoster Review
Authors(s): X.F. Chen, M. Xiao, X. Hou, Y.C. Xu
Authors Affiliations(s): Peking Union Medical College Hospital, China
Background:
The emergence of a multidrug-resistant yeast, Candida auris, has drawn attention to the closely related species from the Candida haemulonii complex includeing Candida haemulonii, Candida duobushaemulonii, Candida pseudohaemulonii, and Candida haemulonii var. vulnera. The goal of this study was to investigate the genetic relationships and drug-resistance profiles among isolates of the C. duobushaemulonii from different cities and to examine possible evidence of transmission if it existed.
Methods:The12 Candida duobushaemulonii isolates causing invasive fungal diseases were collected from nine tertiary hospitals through CHIF-NET. We used antifungal susceptibility testing and whole-genome sequencing (WGS) to investigate drug resistance and genetic diversity among isolates of Candida duobushaemulonii from different geographic areas of China.
Results:Between 2010 and 2017, 12 isolates of Candida duobushaemulonii from China; In the China, 41.7% of Candida duobushaemulonii was isolated from blood, and other invasive sites (Table 1). Phylogenetic analysis using SNPs called against the Candida duobushaemulonii reference strain B09383. Genetic relationships among Candida duobushaemulonii isolates are shown in (Figure 1). The average pairwise difference between the isolates was 691 SNPs (range 15–1273), and there was distinct phylogeographic population structure. Three isolates, F4444, F4464 were different from each other by fewer than 15 SNPs. These two isolates were recovered from the same patients. In addition, two isolates, F4490, F4560, differed by fewer than 90 SNPs recovered from difference hospital. We observed high levels of susceptibility to amphotericin B among 12 tested Candida duobushaemulonii isolates: all had elevated MICs from 4μg/mL to >8μg/ ml. All isolates had elevated MICs of fluconazole ranging from 64 to 256μg/ml (Table 2). Interesting, these isolates don’t have the known mutation in ERG11, only with synonymous mutation.
Conclusions:Our results indicate that, although we are not observing the widespread of the Candida duobushaemulonii, at least these isolates had elevated MICs of fluconazole. We need to investigated the antifungal-resistance mechanism.
Keyword(s): Candida duobushaemulonii, multidrug-resistant yeast, whole-genome sequencingSession Type: 1-hour ePoster Review
Session Title: 1-hour ePoster Review
Authors(s): X.F. Chen, M. Xiao, X. Hou, Y.C. Xu
Authors Affiliations(s): Peking Union Medical College Hospital, China
Background:
The emergence of a multidrug-resistant yeast, Candida auris, has drawn attention to the closely related species from the Candida haemulonii complex includeing Candida haemulonii, Candida duobushaemulonii, Candida pseudohaemulonii, and Candida haemulonii var. vulnera. The goal of this study was to investigate the genetic relationships and drug-resistance profiles among isolates of the C. duobushaemulonii from different cities and to examine possible evidence of transmission if it existed.
Methods:The12 Candida duobushaemulonii isolates causing invasive fungal diseases were collected from nine tertiary hospitals through CHIF-NET. We used antifungal susceptibility testing and whole-genome sequencing (WGS) to investigate drug resistance and genetic diversity among isolates of Candida duobushaemulonii from different geographic areas of China.
Results:Between 2010 and 2017, 12 isolates of Candida duobushaemulonii from China; In the China, 41.7% of Candida duobushaemulonii was isolated from blood, and other invasive sites (Table 1). Phylogenetic analysis using SNPs called against the Candida duobushaemulonii reference strain B09383. Genetic relationships among Candida duobushaemulonii isolates are shown in (Figure 1). The average pairwise difference between the isolates was 691 SNPs (range 15–1273), and there was distinct phylogeographic population structure. Three isolates, F4444, F4464 were different from each other by fewer than 15 SNPs. These two isolates were recovered from the same patients. In addition, two isolates, F4490, F4560, differed by fewer than 90 SNPs recovered from difference hospital. We observed high levels of susceptibility to amphotericin B among 12 tested Candida duobushaemulonii isolates: all had elevated MICs from 4μg/mL to >8μg/ ml. All isolates had elevated MICs of fluconazole ranging from 64 to 256μg/ml (Table 2). Interesting, these isolates don’t have the known mutation in ERG11, only with synonymous mutation.
Conclusions:Our results indicate that, although we are not observing the widespread of the Candida duobushaemulonii, at least these isolates had elevated MICs of fluconazole. We need to investigated the antifungal-resistance mechanism.
Keyword(s): Candida duobushaemulonii, multidrug-resistant yeast, whole-genome sequencing