Session Type: ePosters
Session Title: ePosters
Authors(s): M. Urrutikoetxea-Gutierrez, D. Fernandez-Vecilla, M. Imaz Perez, P. Liendo-Arenzana, L. Hernandez Ragpa, J.A. Álava Menica, J.L. Diaz De Tuesta
Authors Affiliations(s): Basurto University Hospital, Spain
Background:
Until recently the syphilis diagnostic relied mostly on serological methods, even if direct visualization is obviously possible, dark field microscopy is not often available at clinical microbiology laboratories. With the rise of the syndromic commercial molecular assays there is a new chance to complement the nowadays gold-standard indirect diagnostic. The aim of this study is to analyse the importance of the early detection of Treponema pallidum at genital ulcers and other anatomical locations, especially in patients with negative serology.
Methods:We retrieved every sample analysed with the AllplexTM Genital ulcer Assay (Seegene, Korea), a multiplex assay that detects Cytomegalovirus, Haemophilus ducreyi, Herpes simplex virus type 1 and 2, Lymphogranuloma venereum, Treponema pallidum and Varicella-zoster virus from January 2019 to February 2021
We compared every sample positive to Treponema pallidum and with the results of the routine serology conducted at our centre (IgM EIA, IgG/IgM CLIA, FTA-Abs and RPR).
Results:Out of the 1327 samples of 972 patients 111 samples from 108 patients were positive for T. pallidum. Most of the patients were male (104/108) HIV negative (84.2%) and the mean age was 41 years old. Most skin lesions were located at the penis (64.5%) followed by rectal (20%) and oral cavity lesions (14%), there were also two lesions at the vulva (1.8%) and one abdominal region. 94 patients (87%) had at the time of the diagnose a positive serology, and their median RPR value was 1/16. There were three coinfections with herpes and one with Cytomegalovirus.
Conclusions:The PCR is a fast and reliable method for early diagnostic of primary Syphilis, specially at early stages when dealing with possible window periods.
In our two-year series the genital region remains the primary location, this might be biased due to a higher diagnostic suspicion leading to taking samples from ulcers at this location.
More studies, including the molecular typing of this pathogens, must be done in order to fully understand the importance of early detection of T. pallidum, specially at extragenital locations. This can only be done by diagnosing the genital ulcers with this kind of molecular assays
Keyword(s): Syphilis, Genital Ulcer, Syndromic panelSession Type: ePosters
Session Title: ePosters
Authors(s): M. Urrutikoetxea-Gutierrez, D. Fernandez-Vecilla, M. Imaz Perez, P. Liendo-Arenzana, L. Hernandez Ragpa, J.A. Álava Menica, J.L. Diaz De Tuesta
Authors Affiliations(s): Basurto University Hospital, Spain
Background:
Until recently the syphilis diagnostic relied mostly on serological methods, even if direct visualization is obviously possible, dark field microscopy is not often available at clinical microbiology laboratories. With the rise of the syndromic commercial molecular assays there is a new chance to complement the nowadays gold-standard indirect diagnostic. The aim of this study is to analyse the importance of the early detection of Treponema pallidum at genital ulcers and other anatomical locations, especially in patients with negative serology.
Methods:We retrieved every sample analysed with the AllplexTM Genital ulcer Assay (Seegene, Korea), a multiplex assay that detects Cytomegalovirus, Haemophilus ducreyi, Herpes simplex virus type 1 and 2, Lymphogranuloma venereum, Treponema pallidum and Varicella-zoster virus from January 2019 to February 2021
We compared every sample positive to Treponema pallidum and with the results of the routine serology conducted at our centre (IgM EIA, IgG/IgM CLIA, FTA-Abs and RPR).
Results:Out of the 1327 samples of 972 patients 111 samples from 108 patients were positive for T. pallidum. Most of the patients were male (104/108) HIV negative (84.2%) and the mean age was 41 years old. Most skin lesions were located at the penis (64.5%) followed by rectal (20%) and oral cavity lesions (14%), there were also two lesions at the vulva (1.8%) and one abdominal region. 94 patients (87%) had at the time of the diagnose a positive serology, and their median RPR value was 1/16. There were three coinfections with herpes and one with Cytomegalovirus.
Conclusions:The PCR is a fast and reliable method for early diagnostic of primary Syphilis, specially at early stages when dealing with possible window periods.
In our two-year series the genital region remains the primary location, this might be biased due to a higher diagnostic suspicion leading to taking samples from ulcers at this location.
More studies, including the molecular typing of this pathogens, must be done in order to fully understand the importance of early detection of T. pallidum, specially at extragenital locations. This can only be done by diagnosing the genital ulcers with this kind of molecular assays
Keyword(s): Syphilis, Genital Ulcer, Syndromic panel