Session Type: ePosters
Session Title: ePosters
Authors(s): M. Henderikx, M. Oosting, J. De Beer, A. Van Der Zanden, M. Schijffelen
Authors Affiliations(s): Laboratory for Medical Microbiology and Public Health, Netherlands
Background:
Screening for methicillin-resistant Staphylococcus aureus (MRSA) carriage in patients is one of the key factors in the successful control of MRSA in Dutch hospitals. Over the years, rapid molecular detection has evolved as an important diagnostic tool in the detection of MRSA carriers. The aim of this study was to evaluate the diagnostic potential of the Panther Fusion MRSA assay (PFMA) (Hologic) for accurate and rapid detection of MRSA in comparison to conventional culture. The PFMA runs on a fully-automated PCR platform it provides a qualitative MRSA result within 2,5 hours. It also reports distinct ct-values for the orfX-SCCmec junction, mecA/C, GAPDH and internal control targets.
Methods:We performed a prospective study using nasal, throat and perianal swabs in Amies transport medium (eSwab, Copan) collected for MRSA screening. The PFMA was performed both directly from Amies transport medium (direct swab PFMA) and after overnight broth enrichment (broth enriched PFMA). To circumvent the problem of false negative results due to strains with atypical orfX-SCCmec junctions a secondary screening based on the combined mecA/C and GAPDH target (ct-value difference ≤ 5) was introduced. Results were compared to conventional MRSA culture using MRSA2 Brilliance Screening agar (Oxoid) after overnight enrichment in Mueller Hinton 6.5% NaCl broth.
Results:The diagnostic performance for the qualitative detection of MRSA using the PFMA was assessed in 1017 samples from 348 patients. The sensitivity of the broth enriched PFMA was 96% which was considerably higher than the direct swab PFMA (65%). Specificity was high for both the broth enriched PFMA and direct swab PFMA (99% versus 97%). Resulting in a PPV of 85% using the broth enriched PFMA and 77% for the direct swab PFMA. The sensitivity of the broth enriched PFMA could be further increased to 99% by introducing secondary screening of all negative results based on the mecA/C and GAPDH ct-difference. At the down side this lowered the PPV to 63%.
Conclusions:The Panther Fusion MRSA assay suggest a high diagnostic accuracy for MRSA detection in clinical samples after broth enrichment. Resulting in a shorter time-frame of the reporting of the results compared to conventional culture based broth enrichment.
Keyword(s): methicillin- resistant Staphylococcus aureus (MRSA), PCR amplification, broth enrichment cultureCOI Other: Hologic provided financial support for the performance of this evaluation study.
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Henderikx, M. Oosting, J. De Beer, A. Van Der Zanden, M. Schijffelen
Authors Affiliations(s): Laboratory for Medical Microbiology and Public Health, Netherlands
Background:
Screening for methicillin-resistant Staphylococcus aureus (MRSA) carriage in patients is one of the key factors in the successful control of MRSA in Dutch hospitals. Over the years, rapid molecular detection has evolved as an important diagnostic tool in the detection of MRSA carriers. The aim of this study was to evaluate the diagnostic potential of the Panther Fusion MRSA assay (PFMA) (Hologic) for accurate and rapid detection of MRSA in comparison to conventional culture. The PFMA runs on a fully-automated PCR platform it provides a qualitative MRSA result within 2,5 hours. It also reports distinct ct-values for the orfX-SCCmec junction, mecA/C, GAPDH and internal control targets.
Methods:We performed a prospective study using nasal, throat and perianal swabs in Amies transport medium (eSwab, Copan) collected for MRSA screening. The PFMA was performed both directly from Amies transport medium (direct swab PFMA) and after overnight broth enrichment (broth enriched PFMA). To circumvent the problem of false negative results due to strains with atypical orfX-SCCmec junctions a secondary screening based on the combined mecA/C and GAPDH target (ct-value difference ≤ 5) was introduced. Results were compared to conventional MRSA culture using MRSA2 Brilliance Screening agar (Oxoid) after overnight enrichment in Mueller Hinton 6.5% NaCl broth.
Results:The diagnostic performance for the qualitative detection of MRSA using the PFMA was assessed in 1017 samples from 348 patients. The sensitivity of the broth enriched PFMA was 96% which was considerably higher than the direct swab PFMA (65%). Specificity was high for both the broth enriched PFMA and direct swab PFMA (99% versus 97%). Resulting in a PPV of 85% using the broth enriched PFMA and 77% for the direct swab PFMA. The sensitivity of the broth enriched PFMA could be further increased to 99% by introducing secondary screening of all negative results based on the mecA/C and GAPDH ct-difference. At the down side this lowered the PPV to 63%.
Conclusions:The Panther Fusion MRSA assay suggest a high diagnostic accuracy for MRSA detection in clinical samples after broth enrichment. Resulting in a shorter time-frame of the reporting of the results compared to conventional culture based broth enrichment.
Keyword(s): methicillin- resistant Staphylococcus aureus (MRSA), PCR amplification, broth enrichment cultureCOI Other: Hologic provided financial support for the performance of this evaluation study.
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Henderikx, M. Oosting, J. De Beer, A. Van Der Zanden, M. Schijffelen
Authors Affiliations(s): Laboratory for Medical Microbiology and Public Health, Netherlands
Background:
Screening for methicillin-resistant Staphylococcus aureus (MRSA) carriage in patients is one of the key factors in the successful control of MRSA in Dutch hospitals. Over the years, rapid molecular detection has evolved as an important diagnostic tool in the detection of MRSA carriers. The aim of this study was to evaluate the diagnostic potential of the Panther Fusion MRSA assay (PFMA) (Hologic) for accurate and rapid detection of MRSA in comparison to conventional culture. The PFMA runs on a fully-automated PCR platform it provides a qualitative MRSA result within 2,5 hours. It also reports distinct ct-values for the orfX-SCCmec junction, mecA/C, GAPDH and internal control targets.
Methods:We performed a prospective study using nasal, throat and perianal swabs in Amies transport medium (eSwab, Copan) collected for MRSA screening. The PFMA was performed both directly from Amies transport medium (direct swab PFMA) and after overnight broth enrichment (broth enriched PFMA). To circumvent the problem of false negative results due to strains with atypical orfX-SCCmec junctions a secondary screening based on the combined mecA/C and GAPDH target (ct-value difference ≤ 5) was introduced. Results were compared to conventional MRSA culture using MRSA2 Brilliance Screening agar (Oxoid) after overnight enrichment in Mueller Hinton 6.5% NaCl broth.
Results:The diagnostic performance for the qualitative detection of MRSA using the PFMA was assessed in 1017 samples from 348 patients. The sensitivity of the broth enriched PFMA was 96% which was considerably higher than the direct swab PFMA (65%). Specificity was high for both the broth enriched PFMA and direct swab PFMA (99% versus 97%). Resulting in a PPV of 85% using the broth enriched PFMA and 77% for the direct swab PFMA. The sensitivity of the broth enriched PFMA could be further increased to 99% by introducing secondary screening of all negative results based on the mecA/C and GAPDH ct-difference. At the down side this lowered the PPV to 63%.
Conclusions:The Panther Fusion MRSA assay suggest a high diagnostic accuracy for MRSA detection in clinical samples after broth enrichment. Resulting in a shorter time-frame of the reporting of the results compared to conventional culture based broth enrichment.
Keyword(s): methicillin- resistant Staphylococcus aureus (MRSA), PCR amplification, broth enrichment cultureCOI Other: Hologic provided financial support for the performance of this evaluation study.
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Henderikx, M. Oosting, J. De Beer, A. Van Der Zanden, M. Schijffelen
Authors Affiliations(s): Laboratory for Medical Microbiology and Public Health, Netherlands
Background:
Screening for methicillin-resistant Staphylococcus aureus (MRSA) carriage in patients is one of the key factors in the successful control of MRSA in Dutch hospitals. Over the years, rapid molecular detection has evolved as an important diagnostic tool in the detection of MRSA carriers. The aim of this study was to evaluate the diagnostic potential of the Panther Fusion MRSA assay (PFMA) (Hologic) for accurate and rapid detection of MRSA in comparison to conventional culture. The PFMA runs on a fully-automated PCR platform it provides a qualitative MRSA result within 2,5 hours. It also reports distinct ct-values for the orfX-SCCmec junction, mecA/C, GAPDH and internal control targets.
Methods:We performed a prospective study using nasal, throat and perianal swabs in Amies transport medium (eSwab, Copan) collected for MRSA screening. The PFMA was performed both directly from Amies transport medium (direct swab PFMA) and after overnight broth enrichment (broth enriched PFMA). To circumvent the problem of false negative results due to strains with atypical orfX-SCCmec junctions a secondary screening based on the combined mecA/C and GAPDH target (ct-value difference ≤ 5) was introduced. Results were compared to conventional MRSA culture using MRSA2 Brilliance Screening agar (Oxoid) after overnight enrichment in Mueller Hinton 6.5% NaCl broth.
Results:The diagnostic performance for the qualitative detection of MRSA using the PFMA was assessed in 1017 samples from 348 patients. The sensitivity of the broth enriched PFMA was 96% which was considerably higher than the direct swab PFMA (65%). Specificity was high for both the broth enriched PFMA and direct swab PFMA (99% versus 97%). Resulting in a PPV of 85% using the broth enriched PFMA and 77% for the direct swab PFMA. The sensitivity of the broth enriched PFMA could be further increased to 99% by introducing secondary screening of all negative results based on the mecA/C and GAPDH ct-difference. At the down side this lowered the PPV to 63%.
Conclusions:The Panther Fusion MRSA assay suggest a high diagnostic accuracy for MRSA detection in clinical samples after broth enrichment. Resulting in a shorter time-frame of the reporting of the results compared to conventional culture based broth enrichment.
Keyword(s): methicillin- resistant Staphylococcus aureus (MRSA), PCR amplification, broth enrichment cultureCOI Other: Hologic provided financial support for the performance of this evaluation study.