Session Type: ePosters
Session Title: ePosters
Authors(s): D. Ozbey, S. Akkus, B. Kocazeybek
Authors Affiliations(s): Istanbul University-Cerrahpasa, Department of Medical Microbiology, Turkey
Background:
CagL, a virulence factor used by Helicobacter pylori (H. pylori) to attach to host epithelial cells, is a protein encoded by a chromosomal gene region of approximately 165 bp in the CagPAI region. In studies, it has been suggested that the polymorphisms in the region between the 58–62 amino acids of the 194-amino acid CagL protein, which is called the CagL hypervariable motif (CagLHM), affect the binding affinity of CagL to Integrin α5β1 receptor in host epithelial cells and influence the development of various gastrointestinal diseases. We aimed to evaluate the associations of gastroduodenal pathologies, with the polymorphisms of CagL protein from H. pylori DNAs.
Methods:As a patient group 35 subjects (19 Gastric Cancer (GC) and 16 Duodenal Ulcer (DU) cases); and as a control group 26 Non-Ulcer dyspepsia (NUD) cases were included in this study. Molecular studies done from biopsy specimens. A fragment of 651 bp from gene cagL (hp0539) was amplified by Nested-PCR and then PCR products were sequenced by the Sanger Sequencing method. The obtained nucleotide sequences translated into amino acid sequences using ExPASy Bioinformatics tool.
Results:All H.pylori strains were cagL-positive. The D58 amino acid polymorphism was detected in 16 (84%) cases in the GC group and in four (15.4%) cases in the NUD group, and the estimated risk coefficient was significant (OR = 29, p = 0.0001). While the D58/K59 polymorphism was detected in 12 (63.1%) cases in the GC group, it was found in one (3.85%) case in the NUD group. While a significant difference was found between the groups, the estimated risk coefficient was determined as OR = 42 (p = 0.0001). The DKIGQ polymorphism was detected in ten (52.63%) of the GC cases. D58/K59 and DKIGQ were the most common polymorphisms in the GC and were associated with the vacA genotype s1/m2. The D58/K59 amino acid polymorphism was found to have a significant OR value of 8.9 (p = 0.000366) in multivariate logistic regression analysis.
Conclusions:The D58/K59 polymorphism had a significantly higher estimated risk and independent risk coefficient in GC cases and the risk of GC development is 8.9-times higher with this polymorphism
Keyword(s): Helicobacter pylori, Polymorphisms, Gastroduodenal pathologiesCOI Other: This research is funded by the Istanbul University-Cerrahpasa Research Fund (Project Number: TYL-2019-34090)
Session Type: ePosters
Session Title: ePosters
Authors(s): D. Ozbey, S. Akkus, B. Kocazeybek
Authors Affiliations(s): Istanbul University-Cerrahpasa, Department of Medical Microbiology, Turkey
Background:
CagL, a virulence factor used by Helicobacter pylori (H. pylori) to attach to host epithelial cells, is a protein encoded by a chromosomal gene region of approximately 165 bp in the CagPAI region. In studies, it has been suggested that the polymorphisms in the region between the 58–62 amino acids of the 194-amino acid CagL protein, which is called the CagL hypervariable motif (CagLHM), affect the binding affinity of CagL to Integrin α5β1 receptor in host epithelial cells and influence the development of various gastrointestinal diseases. We aimed to evaluate the associations of gastroduodenal pathologies, with the polymorphisms of CagL protein from H. pylori DNAs.
Methods:As a patient group 35 subjects (19 Gastric Cancer (GC) and 16 Duodenal Ulcer (DU) cases); and as a control group 26 Non-Ulcer dyspepsia (NUD) cases were included in this study. Molecular studies done from biopsy specimens. A fragment of 651 bp from gene cagL (hp0539) was amplified by Nested-PCR and then PCR products were sequenced by the Sanger Sequencing method. The obtained nucleotide sequences translated into amino acid sequences using ExPASy Bioinformatics tool.
Results:All H.pylori strains were cagL-positive. The D58 amino acid polymorphism was detected in 16 (84%) cases in the GC group and in four (15.4%) cases in the NUD group, and the estimated risk coefficient was significant (OR = 29, p = 0.0001). While the D58/K59 polymorphism was detected in 12 (63.1%) cases in the GC group, it was found in one (3.85%) case in the NUD group. While a significant difference was found between the groups, the estimated risk coefficient was determined as OR = 42 (p = 0.0001). The DKIGQ polymorphism was detected in ten (52.63%) of the GC cases. D58/K59 and DKIGQ were the most common polymorphisms in the GC and were associated with the vacA genotype s1/m2. The D58/K59 amino acid polymorphism was found to have a significant OR value of 8.9 (p = 0.000366) in multivariate logistic regression analysis.
Conclusions:The D58/K59 polymorphism had a significantly higher estimated risk and independent risk coefficient in GC cases and the risk of GC development is 8.9-times higher with this polymorphism
Keyword(s): Helicobacter pylori, Polymorphisms, Gastroduodenal pathologiesCOI Other: This research is funded by the Istanbul University-Cerrahpasa Research Fund (Project Number: TYL-2019-34090)
Session Type: ePosters
Session Title: ePosters
Authors(s): D. Ozbey, S. Akkus, B. Kocazeybek
Authors Affiliations(s): Istanbul University-Cerrahpasa, Department of Medical Microbiology, Turkey
Background:
CagL, a virulence factor used by Helicobacter pylori (H. pylori) to attach to host epithelial cells, is a protein encoded by a chromosomal gene region of approximately 165 bp in the CagPAI region. In studies, it has been suggested that the polymorphisms in the region between the 58–62 amino acids of the 194-amino acid CagL protein, which is called the CagL hypervariable motif (CagLHM), affect the binding affinity of CagL to Integrin α5β1 receptor in host epithelial cells and influence the development of various gastrointestinal diseases. We aimed to evaluate the associations of gastroduodenal pathologies, with the polymorphisms of CagL protein from H. pylori DNAs.
Methods:As a patient group 35 subjects (19 Gastric Cancer (GC) and 16 Duodenal Ulcer (DU) cases); and as a control group 26 Non-Ulcer dyspepsia (NUD) cases were included in this study. Molecular studies done from biopsy specimens. A fragment of 651 bp from gene cagL (hp0539) was amplified by Nested-PCR and then PCR products were sequenced by the Sanger Sequencing method. The obtained nucleotide sequences translated into amino acid sequences using ExPASy Bioinformatics tool.
Results:All H.pylori strains were cagL-positive. The D58 amino acid polymorphism was detected in 16 (84%) cases in the GC group and in four (15.4%) cases in the NUD group, and the estimated risk coefficient was significant (OR = 29, p = 0.0001). While the D58/K59 polymorphism was detected in 12 (63.1%) cases in the GC group, it was found in one (3.85%) case in the NUD group. While a significant difference was found between the groups, the estimated risk coefficient was determined as OR = 42 (p = 0.0001). The DKIGQ polymorphism was detected in ten (52.63%) of the GC cases. D58/K59 and DKIGQ were the most common polymorphisms in the GC and were associated with the vacA genotype s1/m2. The D58/K59 amino acid polymorphism was found to have a significant OR value of 8.9 (p = 0.000366) in multivariate logistic regression analysis.
Conclusions:The D58/K59 polymorphism had a significantly higher estimated risk and independent risk coefficient in GC cases and the risk of GC development is 8.9-times higher with this polymorphism
Keyword(s): Helicobacter pylori, Polymorphisms, Gastroduodenal pathologiesCOI Other: This research is funded by the Istanbul University-Cerrahpasa Research Fund (Project Number: TYL-2019-34090)
Session Type: ePosters
Session Title: ePosters
Authors(s): D. Ozbey, S. Akkus, B. Kocazeybek
Authors Affiliations(s): Istanbul University-Cerrahpasa, Department of Medical Microbiology, Turkey
Background:
CagL, a virulence factor used by Helicobacter pylori (H. pylori) to attach to host epithelial cells, is a protein encoded by a chromosomal gene region of approximately 165 bp in the CagPAI region. In studies, it has been suggested that the polymorphisms in the region between the 58–62 amino acids of the 194-amino acid CagL protein, which is called the CagL hypervariable motif (CagLHM), affect the binding affinity of CagL to Integrin α5β1 receptor in host epithelial cells and influence the development of various gastrointestinal diseases. We aimed to evaluate the associations of gastroduodenal pathologies, with the polymorphisms of CagL protein from H. pylori DNAs.
Methods:As a patient group 35 subjects (19 Gastric Cancer (GC) and 16 Duodenal Ulcer (DU) cases); and as a control group 26 Non-Ulcer dyspepsia (NUD) cases were included in this study. Molecular studies done from biopsy specimens. A fragment of 651 bp from gene cagL (hp0539) was amplified by Nested-PCR and then PCR products were sequenced by the Sanger Sequencing method. The obtained nucleotide sequences translated into amino acid sequences using ExPASy Bioinformatics tool.
Results:All H.pylori strains were cagL-positive. The D58 amino acid polymorphism was detected in 16 (84%) cases in the GC group and in four (15.4%) cases in the NUD group, and the estimated risk coefficient was significant (OR = 29, p = 0.0001). While the D58/K59 polymorphism was detected in 12 (63.1%) cases in the GC group, it was found in one (3.85%) case in the NUD group. While a significant difference was found between the groups, the estimated risk coefficient was determined as OR = 42 (p = 0.0001). The DKIGQ polymorphism was detected in ten (52.63%) of the GC cases. D58/K59 and DKIGQ were the most common polymorphisms in the GC and were associated with the vacA genotype s1/m2. The D58/K59 amino acid polymorphism was found to have a significant OR value of 8.9 (p = 0.000366) in multivariate logistic regression analysis.
Conclusions:The D58/K59 polymorphism had a significantly higher estimated risk and independent risk coefficient in GC cases and the risk of GC development is 8.9-times higher with this polymorphism
Keyword(s): Helicobacter pylori, Polymorphisms, Gastroduodenal pathologiesCOI Other: This research is funded by the Istanbul University-Cerrahpasa Research Fund (Project Number: TYL-2019-34090)