Session Type: ePosters
Session Title: ePosters
Authors(s): H. Yousfi, A. Sghir, L. Morin
Authors Affiliations(s): Université Evry, France
Background:
Fungi are considered to be among the main causes of infectious diseases, especially, for immunocompromised patients. Pathogenic fungal species are ubiquitous in the environment with important diversity within wastewater treatment plants (WWTPs). They may constitute a serious threat to human health, since it is showed that fungi are not completely removed by conventional WWTPs processes. In addition, antifungal resistance and emergence of life-threatening fungal species, not previously well identified, characterized or not yet cultivated, make patients treatment very complex. Therefore, it is important to manage and control the source of pathogens contamination, to reduce fungal infections incidence.
Methods:Twenty-three wastewater sludge samples were collected from the Seine-Morée WWTP, started de novo without external sludge inoculation, for 236 consecutive days. Metagenomic DNA was extracted according to Nucleo spin soil DNA kit. The eukaryotic diversity was investigated by PCR amplification of the V9 region of the 18S rRNA gene. The resulting PCR fragments were sequenced on Illumina MiSeq platform at Genoscope. Sequence reads affiliation was performed by blast using SILVA_132_18S database. BIOM files (abundance + taxonomy) were constructed and imported in R (version 3.5.2) for statistical analysis.
Results:A total of 74,432 high-quality sequence reads (345 OTUs) were obtained for the twenty-three samples. These sequences are affiliated with nine different fungal phyla among which a not yet cultivated Cryptomycota phylum was the most predominant making up to 76 % of the total fungal population. Among the potential human fungal genera-containing pathogen species, we counted 45, totalizing 8035 reads. Candida and Pichia were the two most important genera reported for Ascomycota phylum, while Lichtheimia and Rhizopus were observed for Mucoromycota phylum. These genera represent the most persistent fungal pathogens within the WWTP microbiome, over the 236-sampling day.
Conclusions:The overall results of this study highlight that treated wastewater and sludge represent a potential source of pathogenic fungi. Hence, it is important to consider human fungal pathogens during safety evaluation for any agricultural or recreational water use.
COI Institutional Grants: Yes
Session Type: ePosters
Session Title: ePosters
Authors(s): H. Yousfi, A. Sghir, L. Morin
Authors Affiliations(s): Université Evry, France
Background:
Fungi are considered to be among the main causes of infectious diseases, especially, for immunocompromised patients. Pathogenic fungal species are ubiquitous in the environment with important diversity within wastewater treatment plants (WWTPs). They may constitute a serious threat to human health, since it is showed that fungi are not completely removed by conventional WWTPs processes. In addition, antifungal resistance and emergence of life-threatening fungal species, not previously well identified, characterized or not yet cultivated, make patients treatment very complex. Therefore, it is important to manage and control the source of pathogens contamination, to reduce fungal infections incidence.
Methods:Twenty-three wastewater sludge samples were collected from the Seine-Morée WWTP, started de novo without external sludge inoculation, for 236 consecutive days. Metagenomic DNA was extracted according to Nucleo spin soil DNA kit. The eukaryotic diversity was investigated by PCR amplification of the V9 region of the 18S rRNA gene. The resulting PCR fragments were sequenced on Illumina MiSeq platform at Genoscope. Sequence reads affiliation was performed by blast using SILVA_132_18S database. BIOM files (abundance + taxonomy) were constructed and imported in R (version 3.5.2) for statistical analysis.
Results:A total of 74,432 high-quality sequence reads (345 OTUs) were obtained for the twenty-three samples. These sequences are affiliated with nine different fungal phyla among which a not yet cultivated Cryptomycota phylum was the most predominant making up to 76 % of the total fungal population. Among the potential human fungal genera-containing pathogen species, we counted 45, totalizing 8035 reads. Candida and Pichia were the two most important genera reported for Ascomycota phylum, while Lichtheimia and Rhizopus were observed for Mucoromycota phylum. These genera represent the most persistent fungal pathogens within the WWTP microbiome, over the 236-sampling day.
Conclusions:The overall results of this study highlight that treated wastewater and sludge represent a potential source of pathogenic fungi. Hence, it is important to consider human fungal pathogens during safety evaluation for any agricultural or recreational water use.
COI Institutional Grants: Yes