Session Type: ePosters
Session Title: ePosters
Authors(s): A. Morieras (1), M. Wootton (2), E. Pillon (1), C. Franceschi (1)
Authors Affiliations(s): (1) bioMérieux, France, (2) Public Health Wales, United Kingdom
Background:
ETEST® Amoxicillin/Clavulanic Acid (ACC) is a new gradient diffusion strip developed with a fixed concentration of clavulanic acid to be compliant with EUCAST guideline. This study aims to determine the minimum inhibitory concentration (MIC) of Enterobacterales and H. influenzae as compared to ISO-20776-1 broth microdilution reference method (BMD) and anaerobes to the agar dilution (AD) reference method based on the CLSI M11-Ed.09.
Methods:
A set of 410 isolates including 195 Enterobacterales, 117 H. influenzae and 98 anaerobes was tested using ETEST® ACC compared to BMD or AD. Results were analyzed for essential (EA) and category (CA) agreements, major (ME) and very major (VME) error rates using EUCAST 2020 breakpoints.
Results:Results are summarized in the table below. ETEST® ACC performances for Enterobacterales, H. influenzae and anaerobes met ISO acceptance criteria for EA (≥ 90%), CA (≥ 90%), ME (≤ 3%). The criteria for VME (≤ 3%) is met for anaerobes and H. influenzae (oral breakpoint). For Enterobacterales and H. influenzae (intravenous breakpoint), the strict VME rates does not met ISO criteria. However, ETEST® ACC results are all one doubling dilution apart from BMD and are due to the absence of a susceptible increased exposure category defined by EUCAST.
Conclusions:Results of this study supports the accuracy of ETEST® ACC for determining MICs of Enterobacterales, H. influenzae and anaerobes. As such, the ETEST® ACC is considered as substantially equivalent to the reference methods BMD and AD.
Keyword(s): amoxicillin/clavulanic acid, ETEST, Clinical trialSession Type: ePosters
Session Title: ePosters
Authors(s): A. Morieras (1), M. Wootton (2), E. Pillon (1), C. Franceschi (1)
Authors Affiliations(s): (1) bioMérieux, France, (2) Public Health Wales, United Kingdom
Background:
ETEST® Amoxicillin/Clavulanic Acid (ACC) is a new gradient diffusion strip developed with a fixed concentration of clavulanic acid to be compliant with EUCAST guideline. This study aims to determine the minimum inhibitory concentration (MIC) of Enterobacterales and H. influenzae as compared to ISO-20776-1 broth microdilution reference method (BMD) and anaerobes to the agar dilution (AD) reference method based on the CLSI M11-Ed.09.
Methods:
A set of 410 isolates including 195 Enterobacterales, 117 H. influenzae and 98 anaerobes was tested using ETEST® ACC compared to BMD or AD. Results were analyzed for essential (EA) and category (CA) agreements, major (ME) and very major (VME) error rates using EUCAST 2020 breakpoints.
Results:Results are summarized in the table below. ETEST® ACC performances for Enterobacterales, H. influenzae and anaerobes met ISO acceptance criteria for EA (≥ 90%), CA (≥ 90%), ME (≤ 3%). The criteria for VME (≤ 3%) is met for anaerobes and H. influenzae (oral breakpoint). For Enterobacterales and H. influenzae (intravenous breakpoint), the strict VME rates does not met ISO criteria. However, ETEST® ACC results are all one doubling dilution apart from BMD and are due to the absence of a susceptible increased exposure category defined by EUCAST.
Conclusions:Results of this study supports the accuracy of ETEST® ACC for determining MICs of Enterobacterales, H. influenzae and anaerobes. As such, the ETEST® ACC is considered as substantially equivalent to the reference methods BMD and AD.
Keyword(s): amoxicillin/clavulanic acid, ETEST, Clinical trial
Session Type: ePosters
Session Title: ePosters
Authors(s): A. Morieras (1), M. Wootton (2), E. Pillon (1), C. Franceschi (1)
Authors Affiliations(s): (1) bioMérieux, France, (2) Public Health Wales, United Kingdom
Background:
ETEST® Amoxicillin/Clavulanic Acid (ACC) is a new gradient diffusion strip developed with a fixed concentration of clavulanic acid to be compliant with EUCAST guideline. This study aims to determine the minimum inhibitory concentration (MIC) of Enterobacterales and H. influenzae as compared to ISO-20776-1 broth microdilution reference method (BMD) and anaerobes to the agar dilution (AD) reference method based on the CLSI M11-Ed.09.
Methods:
A set of 410 isolates including 195 Enterobacterales, 117 H. influenzae and 98 anaerobes was tested using ETEST® ACC compared to BMD or AD. Results were analyzed for essential (EA) and category (CA) agreements, major (ME) and very major (VME) error rates using EUCAST 2020 breakpoints.
Results:Results are summarized in the table below. ETEST® ACC performances for Enterobacterales, H. influenzae and anaerobes met ISO acceptance criteria for EA (≥ 90%), CA (≥ 90%), ME (≤ 3%). The criteria for VME (≤ 3%) is met for anaerobes and H. influenzae (oral breakpoint). For Enterobacterales and H. influenzae (intravenous breakpoint), the strict VME rates does not met ISO criteria. However, ETEST® ACC results are all one doubling dilution apart from BMD and are due to the absence of a susceptible increased exposure category defined by EUCAST.
Conclusions:Results of this study supports the accuracy of ETEST® ACC for determining MICs of Enterobacterales, H. influenzae and anaerobes. As such, the ETEST® ACC is considered as substantially equivalent to the reference methods BMD and AD.
Keyword(s): amoxicillin/clavulanic acid, ETEST, Clinical trialSession Type: ePosters
Session Title: ePosters
Authors(s): A. Morieras (1), M. Wootton (2), E. Pillon (1), C. Franceschi (1)
Authors Affiliations(s): (1) bioMérieux, France, (2) Public Health Wales, United Kingdom
Background:
ETEST® Amoxicillin/Clavulanic Acid (ACC) is a new gradient diffusion strip developed with a fixed concentration of clavulanic acid to be compliant with EUCAST guideline. This study aims to determine the minimum inhibitory concentration (MIC) of Enterobacterales and H. influenzae as compared to ISO-20776-1 broth microdilution reference method (BMD) and anaerobes to the agar dilution (AD) reference method based on the CLSI M11-Ed.09.
Methods:
A set of 410 isolates including 195 Enterobacterales, 117 H. influenzae and 98 anaerobes was tested using ETEST® ACC compared to BMD or AD. Results were analyzed for essential (EA) and category (CA) agreements, major (ME) and very major (VME) error rates using EUCAST 2020 breakpoints.
Results:Results are summarized in the table below. ETEST® ACC performances for Enterobacterales, H. influenzae and anaerobes met ISO acceptance criteria for EA (≥ 90%), CA (≥ 90%), ME (≤ 3%). The criteria for VME (≤ 3%) is met for anaerobes and H. influenzae (oral breakpoint). For Enterobacterales and H. influenzae (intravenous breakpoint), the strict VME rates does not met ISO criteria. However, ETEST® ACC results are all one doubling dilution apart from BMD and are due to the absence of a susceptible increased exposure category defined by EUCAST.
Conclusions:Results of this study supports the accuracy of ETEST® ACC for determining MICs of Enterobacterales, H. influenzae and anaerobes. As such, the ETEST® ACC is considered as substantially equivalent to the reference methods BMD and AD.
Keyword(s): amoxicillin/clavulanic acid, ETEST, Clinical trial