Session Type: ePosters
Session Title: ePosters
Authors(s): M. Fernandez-Pittol (1, 2), M.R. Monte (3), A. Roman (3), L. Oliver (4), O. González-Moreno (4), G. Tudó (3, 2, 5), J. Gonzalez-Martin (3, 2, 5)
Authors Affiliations(s): (1) Microbiology department-Hospital clinic de Barcelona, Spain, (2) Universitat de Barcelona, Spain, (3) Mycrobiology department-Hospital clinic de Barcelona, Spain, (4) Mycrobiology and parasitology department-Sylab diagnósticos globales, Spain, (5) ISGLOBAL, Spain
Third Party Affiliation: I
Background:
The incidence of Non-Tuberculous Mycobacteria (NTM) infections is increasing. These infections mainly affect patients with inflammatory lung disease such as bronchiectasis, or immunodeficient status. The diagnosis is difficult, and the treatment is not well standardized. The management of these patients requires a combined antibiotic therapy, but in many cases, they show a scarce response to treatment or trend to chronicity. Mycobacterium avium complex, MAC (M. avium, M. intracellulare, M. chimaera) are the most frequently isolated in pulmonary infections. The present study aimed to describe the antimicrobial susceptibility pattern among the species of MAC in clinical isolates from a third-level hospital.
Methods:The susceptibility patter of MAC isolates was obtained over a 7-year period (January 2013-December 2020) from a Microbiology department database. The isolates identification was performed by MALDI-TOF MS and/or by molecular techniques (16s rRNA gene sequencing), and the antimicrobial susceptibility study was performed using a microdilution method (Sensititre, Thermo Fisher Scientific). Statistical analysis comparing the susceptibility profile among the specie was performed with Chi-Square test.
Results:A total of 737 strains of MAC were analysed. Three hundred fifty-one (47.62%) were identified as M. avium and, 386 (52.37%) as M.intracellulare/M.chimaera. Four hundred four were clinical samples: sputum (228), bronchial aspirate (45), cerebral fluid (8), lymph node (15), ascitic liquid (1), bronchioalveolar lavage (7), pleural fluid (1), faecal sample (13), synovial fluid (1), bone marrow (1) and the remained samples were strains from SYNLAB laboratory. Table 1 shows drug susceptibility pattern of all isolates. According to minimum inhibitory concentration (MIC) results the profile of M. intracellulare/M. chimaera isolates were more susceptible that M. avium in this cohort.
Conclusions:In our study M. avium showed a MIC values higher than M. intracellulare/M. chimaera, being statistically significant in front of amikacin, ciprofloxacin, cotrimoxazole, doxycycline, isoniazid, moxifloxacin and rifabutin.
Keyword(s): NTM, Drug-susceptibility, pulmonary infectionSession Type: ePosters
Session Title: ePosters
Authors(s): M. Fernandez-Pittol (1, 2), M.R. Monte (3), A. Roman (3), L. Oliver (4), O. González-Moreno (4), G. Tudó (3, 2, 5), J. Gonzalez-Martin (3, 2, 5)
Authors Affiliations(s): (1) Microbiology department-Hospital clinic de Barcelona, Spain, (2) Universitat de Barcelona, Spain, (3) Mycrobiology department-Hospital clinic de Barcelona, Spain, (4) Mycrobiology and parasitology department-Sylab diagnósticos globales, Spain, (5) ISGLOBAL, Spain
Third Party Affiliation: I
Background:
The incidence of Non-Tuberculous Mycobacteria (NTM) infections is increasing. These infections mainly affect patients with inflammatory lung disease such as bronchiectasis, or immunodeficient status. The diagnosis is difficult, and the treatment is not well standardized. The management of these patients requires a combined antibiotic therapy, but in many cases, they show a scarce response to treatment or trend to chronicity. Mycobacterium avium complex, MAC (M. avium, M. intracellulare, M. chimaera) are the most frequently isolated in pulmonary infections. The present study aimed to describe the antimicrobial susceptibility pattern among the species of MAC in clinical isolates from a third-level hospital.
Methods:The susceptibility patter of MAC isolates was obtained over a 7-year period (January 2013-December 2020) from a Microbiology department database. The isolates identification was performed by MALDI-TOF MS and/or by molecular techniques (16s rRNA gene sequencing), and the antimicrobial susceptibility study was performed using a microdilution method (Sensititre, Thermo Fisher Scientific). Statistical analysis comparing the susceptibility profile among the specie was performed with Chi-Square test.
Results:A total of 737 strains of MAC were analysed. Three hundred fifty-one (47.62%) were identified as M. avium and, 386 (52.37%) as M.intracellulare/M.chimaera. Four hundred four were clinical samples: sputum (228), bronchial aspirate (45), cerebral fluid (8), lymph node (15), ascitic liquid (1), bronchioalveolar lavage (7), pleural fluid (1), faecal sample (13), synovial fluid (1), bone marrow (1) and the remained samples were strains from SYNLAB laboratory. Table 1 shows drug susceptibility pattern of all isolates. According to minimum inhibitory concentration (MIC) results the profile of M. intracellulare/M. chimaera isolates were more susceptible that M. avium in this cohort.
Conclusions:In our study M. avium showed a MIC values higher than M. intracellulare/M. chimaera, being statistically significant in front of amikacin, ciprofloxacin, cotrimoxazole, doxycycline, isoniazid, moxifloxacin and rifabutin.
Keyword(s): NTM, Drug-susceptibility, pulmonary infection
Hospital clinic of Barcelona, ISGLOBAL-Barcelona, Spain, Universitat de Barcelona-Barcelona, Spain
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Fernandez-Pittol (1, 2), M.R. Monte (3), A. Roman (3), L. Oliver (4), O. González-Moreno (4), G. Tudó (3, 2, 5), J. Gonzalez-Martin (3, 2, 5)
Authors Affiliations(s): (1) Microbiology department-Hospital clinic de Barcelona, Spain, (2) Universitat de Barcelona, Spain, (3) Mycrobiology department-Hospital clinic de Barcelona, Spain, (4) Mycrobiology and parasitology department-Sylab diagnósticos globales, Spain, (5) ISGLOBAL, Spain
Third Party Affiliation: I
Background:
The incidence of Non-Tuberculous Mycobacteria (NTM) infections is increasing. These infections mainly affect patients with inflammatory lung disease such as bronchiectasis, or immunodeficient status. The diagnosis is difficult, and the treatment is not well standardized. The management of these patients requires a combined antibiotic therapy, but in many cases, they show a scarce response to treatment or trend to chronicity. Mycobacterium avium complex, MAC (M. avium, M. intracellulare, M. chimaera) are the most frequently isolated in pulmonary infections. The present study aimed to describe the antimicrobial susceptibility pattern among the species of MAC in clinical isolates from a third-level hospital.
Methods:The susceptibility patter of MAC isolates was obtained over a 7-year period (January 2013-December 2020) from a Microbiology department database. The isolates identification was performed by MALDI-TOF MS and/or by molecular techniques (16s rRNA gene sequencing), and the antimicrobial susceptibility study was performed using a microdilution method (Sensititre, Thermo Fisher Scientific). Statistical analysis comparing the susceptibility profile among the specie was performed with Chi-Square test.
Results:A total of 737 strains of MAC were analysed. Three hundred fifty-one (47.62%) were identified as M. avium and, 386 (52.37%) as M.intracellulare/M.chimaera. Four hundred four were clinical samples: sputum (228), bronchial aspirate (45), cerebral fluid (8), lymph node (15), ascitic liquid (1), bronchioalveolar lavage (7), pleural fluid (1), faecal sample (13), synovial fluid (1), bone marrow (1) and the remained samples were strains from SYNLAB laboratory. Table 1 shows drug susceptibility pattern of all isolates. According to minimum inhibitory concentration (MIC) results the profile of M. intracellulare/M. chimaera isolates were more susceptible that M. avium in this cohort.
Conclusions:In our study M. avium showed a MIC values higher than M. intracellulare/M. chimaera, being statistically significant in front of amikacin, ciprofloxacin, cotrimoxazole, doxycycline, isoniazid, moxifloxacin and rifabutin.
Keyword(s): NTM, Drug-susceptibility, pulmonary infectionSession Type: ePosters
Session Title: ePosters
Authors(s): M. Fernandez-Pittol (1, 2), M.R. Monte (3), A. Roman (3), L. Oliver (4), O. González-Moreno (4), G. Tudó (3, 2, 5), J. Gonzalez-Martin (3, 2, 5)
Authors Affiliations(s): (1) Microbiology department-Hospital clinic de Barcelona, Spain, (2) Universitat de Barcelona, Spain, (3) Mycrobiology department-Hospital clinic de Barcelona, Spain, (4) Mycrobiology and parasitology department-Sylab diagnósticos globales, Spain, (5) ISGLOBAL, Spain
Third Party Affiliation: I
Background:
The incidence of Non-Tuberculous Mycobacteria (NTM) infections is increasing. These infections mainly affect patients with inflammatory lung disease such as bronchiectasis, or immunodeficient status. The diagnosis is difficult, and the treatment is not well standardized. The management of these patients requires a combined antibiotic therapy, but in many cases, they show a scarce response to treatment or trend to chronicity. Mycobacterium avium complex, MAC (M. avium, M. intracellulare, M. chimaera) are the most frequently isolated in pulmonary infections. The present study aimed to describe the antimicrobial susceptibility pattern among the species of MAC in clinical isolates from a third-level hospital.
Methods:The susceptibility patter of MAC isolates was obtained over a 7-year period (January 2013-December 2020) from a Microbiology department database. The isolates identification was performed by MALDI-TOF MS and/or by molecular techniques (16s rRNA gene sequencing), and the antimicrobial susceptibility study was performed using a microdilution method (Sensititre, Thermo Fisher Scientific). Statistical analysis comparing the susceptibility profile among the specie was performed with Chi-Square test.
Results:A total of 737 strains of MAC were analysed. Three hundred fifty-one (47.62%) were identified as M. avium and, 386 (52.37%) as M.intracellulare/M.chimaera. Four hundred four were clinical samples: sputum (228), bronchial aspirate (45), cerebral fluid (8), lymph node (15), ascitic liquid (1), bronchioalveolar lavage (7), pleural fluid (1), faecal sample (13), synovial fluid (1), bone marrow (1) and the remained samples were strains from SYNLAB laboratory. Table 1 shows drug susceptibility pattern of all isolates. According to minimum inhibitory concentration (MIC) results the profile of M. intracellulare/M. chimaera isolates were more susceptible that M. avium in this cohort.
Conclusions:In our study M. avium showed a MIC values higher than M. intracellulare/M. chimaera, being statistically significant in front of amikacin, ciprofloxacin, cotrimoxazole, doxycycline, isoniazid, moxifloxacin and rifabutin.
Keyword(s): NTM, Drug-susceptibility, pulmonary infection