Session Type: ePosters
Session Title: ePosters
Authors(s): Z. Athanasakopoulou (1), K. Tsilipounidaki (2), M. Sofia (1), D.C. Chatzopoulos (1), A. Giannakopoulos (1), V. Spyrou (3), A. Touloudi (1), M. Satra (4), D. Galamatis (5), V. Diamantopoulos (6), S. Mpellou (7), E. Petinaki (2), C. Billinis (1, 4)
Authors Affiliations(s): (1) Faculty of Veterinary Science, University of Thessaly, Greece, (2) Faculty of Medicine, University of Thessaly, Greece, (3) Faculty of Animal Science, University of Thessaly, Greece, (4) Faculty of Public and Integrated Health, University of Thessaly, Greece, (5) Hellenic Agricultural Organization DIMITRA (ELGO DIMITRA), Greece, (6) Directorate of Public Health, Prefecture of Peloponnese, Greece, (7) Bioefarmoges Eleftheriou LP -Integrated Mosquito Control, Greece
Background:
Wild birds have been documented as reservoirs and spreaders of extended-spectrum cephalosporin resistant (ESCR) bacteria, and as sentinels of antimicrobial resistance. This study aimed to evaluate the occurrence of ESBL/AmpC-producing strains in various wild bird species from Greece and to determine their resistance phenotype.
Methods:A total of 168 cloacal swab samples were collected from thirty different wild bird species. For the detection of ESCR and ESBL/AmpC-producing isolates, swabs were directly streaked on CHROMID® ESBL agar (BioMérieux, Marcy l’Etoile, France) and all morphologically different colonies were subcultured on MacConkey agar. Identification and antimicrobial susceptibility testing of the obtained strains were performed using the Vitek-2 system (BioMérieux, Marcy l’Etoile, France). Isolates that presented resistance to 3rd generation cephalosporins were screened for ESBL production by the double disk synergy test and/or the combination disk test, as well as for AmpC production using Etest strips (Liofilchem).
Results:Sixty-seven ESCR Enterobacterales and twenty-seven ESCR non-fermentative Gram-negative bacteria were isolated from 83 (49.4%) samples of 20 wild bird species. Nine E. coli strains were phenotypically confirmed to produce ESBL and one to produce AmpC. All the ESBL/AmpC-producing E. coli additionally exhibited resistance to fluoroquinolones, tetracycline and trimethoprim/sulfamethoxazole. Six of the ESBL-producing E. coli isolates as well as the AmpC-producing strain were obtained from Eurasian magpies (Pica pica), while the remaining three E. coli ESBL producers were retrieved from a Short-toed snake eagle (Circaetus gallicus), a Eurasian scops owl (Otus scops) and a Common swift (Apus apus). The ESBL phenotype was also detected in a Pseudomonas fluorescens and a strain of the Acinetobacter baumannii complex, which were obtained from a Eurasian scops owl (Otus scops) and a Common blackbird (Turdus merula), respectively. The Pseudomonas fluorescens isolate presented resistance to fluoroquinolones and tetracycline whereas the strain of the Acinetobacter baumannii complex was susceptible to all the tested non β-lactam antibiotics.
Conclusions:This study assessed the occurrence and the resistance phenotype of ESBL/AmpC-producing fecal bacteria isolated from various wild birds in Greece. Our results provide evidence of the dissemination of strains that are resistant to critically important antimicrobials, a fact that raises concern in a One Health perspective.
COI Other: This work has been co-funded by the European Union and the General Secretariat for Research and Innovation, Ministry of Development & Investments, under the project «Novel technologies for surveillance and characterization of Extended-spectrum β-lactamase and Carbapenemase producing Enterobacteriaceae, in humans and animals (CARBATECH)» T2DGE-0944, of the Bilateral S&T Cooperation Program Greece – Germany 2017. This support is gratefully acknowledged.
Session Type: ePosters
Session Title: ePosters
Authors(s): Z. Athanasakopoulou (1), K. Tsilipounidaki (2), M. Sofia (1), D.C. Chatzopoulos (1), A. Giannakopoulos (1), V. Spyrou (3), A. Touloudi (1), M. Satra (4), D. Galamatis (5), V. Diamantopoulos (6), S. Mpellou (7), E. Petinaki (2), C. Billinis (1, 4)
Authors Affiliations(s): (1) Faculty of Veterinary Science, University of Thessaly, Greece, (2) Faculty of Medicine, University of Thessaly, Greece, (3) Faculty of Animal Science, University of Thessaly, Greece, (4) Faculty of Public and Integrated Health, University of Thessaly, Greece, (5) Hellenic Agricultural Organization DIMITRA (ELGO DIMITRA), Greece, (6) Directorate of Public Health, Prefecture of Peloponnese, Greece, (7) Bioefarmoges Eleftheriou LP -Integrated Mosquito Control, Greece
Background:
Wild birds have been documented as reservoirs and spreaders of extended-spectrum cephalosporin resistant (ESCR) bacteria, and as sentinels of antimicrobial resistance. This study aimed to evaluate the occurrence of ESBL/AmpC-producing strains in various wild bird species from Greece and to determine their resistance phenotype.
Methods:A total of 168 cloacal swab samples were collected from thirty different wild bird species. For the detection of ESCR and ESBL/AmpC-producing isolates, swabs were directly streaked on CHROMID® ESBL agar (BioMérieux, Marcy l’Etoile, France) and all morphologically different colonies were subcultured on MacConkey agar. Identification and antimicrobial susceptibility testing of the obtained strains were performed using the Vitek-2 system (BioMérieux, Marcy l’Etoile, France). Isolates that presented resistance to 3rd generation cephalosporins were screened for ESBL production by the double disk synergy test and/or the combination disk test, as well as for AmpC production using Etest strips (Liofilchem).
Results:Sixty-seven ESCR Enterobacterales and twenty-seven ESCR non-fermentative Gram-negative bacteria were isolated from 83 (49.4%) samples of 20 wild bird species. Nine E. coli strains were phenotypically confirmed to produce ESBL and one to produce AmpC. All the ESBL/AmpC-producing E. coli additionally exhibited resistance to fluoroquinolones, tetracycline and trimethoprim/sulfamethoxazole. Six of the ESBL-producing E. coli isolates as well as the AmpC-producing strain were obtained from Eurasian magpies (Pica pica), while the remaining three E. coli ESBL producers were retrieved from a Short-toed snake eagle (Circaetus gallicus), a Eurasian scops owl (Otus scops) and a Common swift (Apus apus). The ESBL phenotype was also detected in a Pseudomonas fluorescens and a strain of the Acinetobacter baumannii complex, which were obtained from a Eurasian scops owl (Otus scops) and a Common blackbird (Turdus merula), respectively. The Pseudomonas fluorescens isolate presented resistance to fluoroquinolones and tetracycline whereas the strain of the Acinetobacter baumannii complex was susceptible to all the tested non β-lactam antibiotics.
Conclusions:This study assessed the occurrence and the resistance phenotype of ESBL/AmpC-producing fecal bacteria isolated from various wild birds in Greece. Our results provide evidence of the dissemination of strains that are resistant to critically important antimicrobials, a fact that raises concern in a One Health perspective.
COI Other: This work has been co-funded by the European Union and the General Secretariat for Research and Innovation, Ministry of Development & Investments, under the project «Novel technologies for surveillance and characterization of Extended-spectrum β-lactamase and Carbapenemase producing Enterobacteriaceae, in humans and animals (CARBATECH)» T2DGE-0944, of the Bilateral S&T Cooperation Program Greece – Germany 2017. This support is gratefully acknowledged.
Session Type: ePosters
Session Title: ePosters
Authors(s): Z. Athanasakopoulou (1), K. Tsilipounidaki (2), M. Sofia (1), D.C. Chatzopoulos (1), A. Giannakopoulos (1), V. Spyrou (3), A. Touloudi (1), M. Satra (4), D. Galamatis (5), V. Diamantopoulos (6), S. Mpellou (7), E. Petinaki (2), C. Billinis (1, 4)
Authors Affiliations(s): (1) Faculty of Veterinary Science, University of Thessaly, Greece, (2) Faculty of Medicine, University of Thessaly, Greece, (3) Faculty of Animal Science, University of Thessaly, Greece, (4) Faculty of Public and Integrated Health, University of Thessaly, Greece, (5) Hellenic Agricultural Organization DIMITRA (ELGO DIMITRA), Greece, (6) Directorate of Public Health, Prefecture of Peloponnese, Greece, (7) Bioefarmoges Eleftheriou LP -Integrated Mosquito Control, Greece
Background:
Wild birds have been documented as reservoirs and spreaders of extended-spectrum cephalosporin resistant (ESCR) bacteria, and as sentinels of antimicrobial resistance. This study aimed to evaluate the occurrence of ESBL/AmpC-producing strains in various wild bird species from Greece and to determine their resistance phenotype.
Methods:A total of 168 cloacal swab samples were collected from thirty different wild bird species. For the detection of ESCR and ESBL/AmpC-producing isolates, swabs were directly streaked on CHROMID® ESBL agar (BioMérieux, Marcy l’Etoile, France) and all morphologically different colonies were subcultured on MacConkey agar. Identification and antimicrobial susceptibility testing of the obtained strains were performed using the Vitek-2 system (BioMérieux, Marcy l’Etoile, France). Isolates that presented resistance to 3rd generation cephalosporins were screened for ESBL production by the double disk synergy test and/or the combination disk test, as well as for AmpC production using Etest strips (Liofilchem).
Results:Sixty-seven ESCR Enterobacterales and twenty-seven ESCR non-fermentative Gram-negative bacteria were isolated from 83 (49.4%) samples of 20 wild bird species. Nine E. coli strains were phenotypically confirmed to produce ESBL and one to produce AmpC. All the ESBL/AmpC-producing E. coli additionally exhibited resistance to fluoroquinolones, tetracycline and trimethoprim/sulfamethoxazole. Six of the ESBL-producing E. coli isolates as well as the AmpC-producing strain were obtained from Eurasian magpies (Pica pica), while the remaining three E. coli ESBL producers were retrieved from a Short-toed snake eagle (Circaetus gallicus), a Eurasian scops owl (Otus scops) and a Common swift (Apus apus). The ESBL phenotype was also detected in a Pseudomonas fluorescens and a strain of the Acinetobacter baumannii complex, which were obtained from a Eurasian scops owl (Otus scops) and a Common blackbird (Turdus merula), respectively. The Pseudomonas fluorescens isolate presented resistance to fluoroquinolones and tetracycline whereas the strain of the Acinetobacter baumannii complex was susceptible to all the tested non β-lactam antibiotics.
Conclusions:This study assessed the occurrence and the resistance phenotype of ESBL/AmpC-producing fecal bacteria isolated from various wild birds in Greece. Our results provide evidence of the dissemination of strains that are resistant to critically important antimicrobials, a fact that raises concern in a One Health perspective.
COI Other: This work has been co-funded by the European Union and the General Secretariat for Research and Innovation, Ministry of Development & Investments, under the project «Novel technologies for surveillance and characterization of Extended-spectrum β-lactamase and Carbapenemase producing Enterobacteriaceae, in humans and animals (CARBATECH)» T2DGE-0944, of the Bilateral S&T Cooperation Program Greece – Germany 2017. This support is gratefully acknowledged.
Session Type: ePosters
Session Title: ePosters
Authors(s): Z. Athanasakopoulou (1), K. Tsilipounidaki (2), M. Sofia (1), D.C. Chatzopoulos (1), A. Giannakopoulos (1), V. Spyrou (3), A. Touloudi (1), M. Satra (4), D. Galamatis (5), V. Diamantopoulos (6), S. Mpellou (7), E. Petinaki (2), C. Billinis (1, 4)
Authors Affiliations(s): (1) Faculty of Veterinary Science, University of Thessaly, Greece, (2) Faculty of Medicine, University of Thessaly, Greece, (3) Faculty of Animal Science, University of Thessaly, Greece, (4) Faculty of Public and Integrated Health, University of Thessaly, Greece, (5) Hellenic Agricultural Organization DIMITRA (ELGO DIMITRA), Greece, (6) Directorate of Public Health, Prefecture of Peloponnese, Greece, (7) Bioefarmoges Eleftheriou LP -Integrated Mosquito Control, Greece
Background:
Wild birds have been documented as reservoirs and spreaders of extended-spectrum cephalosporin resistant (ESCR) bacteria, and as sentinels of antimicrobial resistance. This study aimed to evaluate the occurrence of ESBL/AmpC-producing strains in various wild bird species from Greece and to determine their resistance phenotype.
Methods:A total of 168 cloacal swab samples were collected from thirty different wild bird species. For the detection of ESCR and ESBL/AmpC-producing isolates, swabs were directly streaked on CHROMID® ESBL agar (BioMérieux, Marcy l’Etoile, France) and all morphologically different colonies were subcultured on MacConkey agar. Identification and antimicrobial susceptibility testing of the obtained strains were performed using the Vitek-2 system (BioMérieux, Marcy l’Etoile, France). Isolates that presented resistance to 3rd generation cephalosporins were screened for ESBL production by the double disk synergy test and/or the combination disk test, as well as for AmpC production using Etest strips (Liofilchem).
Results:Sixty-seven ESCR Enterobacterales and twenty-seven ESCR non-fermentative Gram-negative bacteria were isolated from 83 (49.4%) samples of 20 wild bird species. Nine E. coli strains were phenotypically confirmed to produce ESBL and one to produce AmpC. All the ESBL/AmpC-producing E. coli additionally exhibited resistance to fluoroquinolones, tetracycline and trimethoprim/sulfamethoxazole. Six of the ESBL-producing E. coli isolates as well as the AmpC-producing strain were obtained from Eurasian magpies (Pica pica), while the remaining three E. coli ESBL producers were retrieved from a Short-toed snake eagle (Circaetus gallicus), a Eurasian scops owl (Otus scops) and a Common swift (Apus apus). The ESBL phenotype was also detected in a Pseudomonas fluorescens and a strain of the Acinetobacter baumannii complex, which were obtained from a Eurasian scops owl (Otus scops) and a Common blackbird (Turdus merula), respectively. The Pseudomonas fluorescens isolate presented resistance to fluoroquinolones and tetracycline whereas the strain of the Acinetobacter baumannii complex was susceptible to all the tested non β-lactam antibiotics.
Conclusions:This study assessed the occurrence and the resistance phenotype of ESBL/AmpC-producing fecal bacteria isolated from various wild birds in Greece. Our results provide evidence of the dissemination of strains that are resistant to critically important antimicrobials, a fact that raises concern in a One Health perspective.
COI Other: This work has been co-funded by the European Union and the General Secretariat for Research and Innovation, Ministry of Development & Investments, under the project «Novel technologies for surveillance and characterization of Extended-spectrum β-lactamase and Carbapenemase producing Enterobacteriaceae, in humans and animals (CARBATECH)» T2DGE-0944, of the Bilateral S&T Cooperation Program Greece – Germany 2017. This support is gratefully acknowledged.