Session Type: ePosters
Session Title: ePosters
Authors(s): B. Schmid (1), A. Kuenstner (2), A. Faehnrich (2), H. Busch (2), M. Glatz (1), P. Bosshard (1)
Authors Affiliations(s): (1) Department of Dermatology, University Hospital Zurich, University of Zurich, Raemistrasse 100, Switzerland, (2) Institute of Experimental Dermatology, University of Luebeck, Ratzeburger Allee 160, Germany
Background:
The skin is the largest organ of the human body and the first-line defense against attacking pathogens. In addition, beneficial microorganisms on the skin protect our body by preventing pathogen intrusion. Indeed, dysbiosis and microbiota instability can contribute to skin diseases such as atopic dermatitis. Bacterial communities are well known to remain largely stable over time, but little is known about fungi. Here, we aim to improve the understanding of the stability of the fungal microbiota on skin in healthy subjects.
Methods:Skin swabs of 11 healthy subjects were taken monthly from 4 skin sites (antecubital crease, glabella, vertex, and dorsal neck) for the time span of a year. Libraries were prepared and sequenced with amplicon-based next-generation sequencing (NGS) of the fungal ITS1 region. Relative abundance, α- (Shannon diversity index) and ß-diversity were calculated to examine the influence of seasons.
Results:The fungal microbiota on skin was dominated by the class Malasseziomycetes (Figure 1) at all skin sites. Malassezia restricta was the single most abundant Malassezia species followed by M. globosa and M. sympodialis with the latter two having a high relative abundance at the dorsal neck or varying in abundance between individuals, respectively. Over the period of one year the relative abundances of fungal classes and species remained stable with some outliers mainly among non-Malassezia fungi. Analyses of the α- and ß-diversity supported the stability throughout the year whereas significant differences between individuals were found (data not shown).
Conclusions:The predominant class Malasseziomycetes and the most abundant Malassezia species are highly stable over time within individuals and within the study group, while the relative abundance of less frequent fungal classes may change. In contrast, we found significant inter-individual differences of the fungal communities. Thus, although many factors can transiently disturb the fungal microbiota on the skin and despite fluctuations of low abundant classes, we conclude that the fungal community forms a temporally robust and individual fingerprint in healthy subjects.
Keyword(s): fungal skin microbiota, longitudinal study, healthy individualsSession Type: ePosters
Session Title: ePosters
Authors(s): B. Schmid (1), A. Kuenstner (2), A. Faehnrich (2), H. Busch (2), M. Glatz (1), P. Bosshard (1)
Authors Affiliations(s): (1) Department of Dermatology, University Hospital Zurich, University of Zurich, Raemistrasse 100, Switzerland, (2) Institute of Experimental Dermatology, University of Luebeck, Ratzeburger Allee 160, Germany
Background:
The skin is the largest organ of the human body and the first-line defense against attacking pathogens. In addition, beneficial microorganisms on the skin protect our body by preventing pathogen intrusion. Indeed, dysbiosis and microbiota instability can contribute to skin diseases such as atopic dermatitis. Bacterial communities are well known to remain largely stable over time, but little is known about fungi. Here, we aim to improve the understanding of the stability of the fungal microbiota on skin in healthy subjects.
Methods:Skin swabs of 11 healthy subjects were taken monthly from 4 skin sites (antecubital crease, glabella, vertex, and dorsal neck) for the time span of a year. Libraries were prepared and sequenced with amplicon-based next-generation sequencing (NGS) of the fungal ITS1 region. Relative abundance, α- (Shannon diversity index) and ß-diversity were calculated to examine the influence of seasons.
Results:The fungal microbiota on skin was dominated by the class Malasseziomycetes (Figure 1) at all skin sites. Malassezia restricta was the single most abundant Malassezia species followed by M. globosa and M. sympodialis with the latter two having a high relative abundance at the dorsal neck or varying in abundance between individuals, respectively. Over the period of one year the relative abundances of fungal classes and species remained stable with some outliers mainly among non-Malassezia fungi. Analyses of the α- and ß-diversity supported the stability throughout the year whereas significant differences between individuals were found (data not shown).
Conclusions:The predominant class Malasseziomycetes and the most abundant Malassezia species are highly stable over time within individuals and within the study group, while the relative abundance of less frequent fungal classes may change. In contrast, we found significant inter-individual differences of the fungal communities. Thus, although many factors can transiently disturb the fungal microbiota on the skin and despite fluctuations of low abundant classes, we conclude that the fungal community forms a temporally robust and individual fingerprint in healthy subjects.
Keyword(s): fungal skin microbiota, longitudinal study, healthy individuals
Session Type: ePosters
Session Title: ePosters
Authors(s): B. Schmid (1), A. Kuenstner (2), A. Faehnrich (2), H. Busch (2), M. Glatz (1), P. Bosshard (1)
Authors Affiliations(s): (1) Department of Dermatology, University Hospital Zurich, University of Zurich, Raemistrasse 100, Switzerland, (2) Institute of Experimental Dermatology, University of Luebeck, Ratzeburger Allee 160, Germany
Background:
The skin is the largest organ of the human body and the first-line defense against attacking pathogens. In addition, beneficial microorganisms on the skin protect our body by preventing pathogen intrusion. Indeed, dysbiosis and microbiota instability can contribute to skin diseases such as atopic dermatitis. Bacterial communities are well known to remain largely stable over time, but little is known about fungi. Here, we aim to improve the understanding of the stability of the fungal microbiota on skin in healthy subjects.
Methods:Skin swabs of 11 healthy subjects were taken monthly from 4 skin sites (antecubital crease, glabella, vertex, and dorsal neck) for the time span of a year. Libraries were prepared and sequenced with amplicon-based next-generation sequencing (NGS) of the fungal ITS1 region. Relative abundance, α- (Shannon diversity index) and ß-diversity were calculated to examine the influence of seasons.
Results:The fungal microbiota on skin was dominated by the class Malasseziomycetes (Figure 1) at all skin sites. Malassezia restricta was the single most abundant Malassezia species followed by M. globosa and M. sympodialis with the latter two having a high relative abundance at the dorsal neck or varying in abundance between individuals, respectively. Over the period of one year the relative abundances of fungal classes and species remained stable with some outliers mainly among non-Malassezia fungi. Analyses of the α- and ß-diversity supported the stability throughout the year whereas significant differences between individuals were found (data not shown).
Conclusions:The predominant class Malasseziomycetes and the most abundant Malassezia species are highly stable over time within individuals and within the study group, while the relative abundance of less frequent fungal classes may change. In contrast, we found significant inter-individual differences of the fungal communities. Thus, although many factors can transiently disturb the fungal microbiota on the skin and despite fluctuations of low abundant classes, we conclude that the fungal community forms a temporally robust and individual fingerprint in healthy subjects.
Keyword(s): fungal skin microbiota, longitudinal study, healthy individualsSession Type: ePosters
Session Title: ePosters
Authors(s): B. Schmid (1), A. Kuenstner (2), A. Faehnrich (2), H. Busch (2), M. Glatz (1), P. Bosshard (1)
Authors Affiliations(s): (1) Department of Dermatology, University Hospital Zurich, University of Zurich, Raemistrasse 100, Switzerland, (2) Institute of Experimental Dermatology, University of Luebeck, Ratzeburger Allee 160, Germany
Background:
The skin is the largest organ of the human body and the first-line defense against attacking pathogens. In addition, beneficial microorganisms on the skin protect our body by preventing pathogen intrusion. Indeed, dysbiosis and microbiota instability can contribute to skin diseases such as atopic dermatitis. Bacterial communities are well known to remain largely stable over time, but little is known about fungi. Here, we aim to improve the understanding of the stability of the fungal microbiota on skin in healthy subjects.
Methods:Skin swabs of 11 healthy subjects were taken monthly from 4 skin sites (antecubital crease, glabella, vertex, and dorsal neck) for the time span of a year. Libraries were prepared and sequenced with amplicon-based next-generation sequencing (NGS) of the fungal ITS1 region. Relative abundance, α- (Shannon diversity index) and ß-diversity were calculated to examine the influence of seasons.
Results:The fungal microbiota on skin was dominated by the class Malasseziomycetes (Figure 1) at all skin sites. Malassezia restricta was the single most abundant Malassezia species followed by M. globosa and M. sympodialis with the latter two having a high relative abundance at the dorsal neck or varying in abundance between individuals, respectively. Over the period of one year the relative abundances of fungal classes and species remained stable with some outliers mainly among non-Malassezia fungi. Analyses of the α- and ß-diversity supported the stability throughout the year whereas significant differences between individuals were found (data not shown).
Conclusions:The predominant class Malasseziomycetes and the most abundant Malassezia species are highly stable over time within individuals and within the study group, while the relative abundance of less frequent fungal classes may change. In contrast, we found significant inter-individual differences of the fungal communities. Thus, although many factors can transiently disturb the fungal microbiota on the skin and despite fluctuations of low abundant classes, we conclude that the fungal community forms a temporally robust and individual fingerprint in healthy subjects.
Keyword(s): fungal skin microbiota, longitudinal study, healthy individuals