Session Type: ePosters
Session Title: ePosters
Authors(s): R. Lapid (1), Y. Motro (2), B. Khalfin (2), G. Kahila Bar-Gal (1), J. Moran-Gilad (2)
Authors Affiliations(s): (1) The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot 7610001, Israel, Israel, (2) Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel, Israel
Background:
The Golden Jackal (Canis aureus), is a medium canid carnivore which thrives in close proximity to human settlements, making it a potential reservoir for many zoonotic diseases. The GJ is one of the major reservoirs for rabies in Israel but has not been extensively studied. Despite growing research on animal microbiota, little is known regarding the GJ gut microbiota. Therefore, we sought to study the geographical spread of GJ across Rabies hot spots and investigate its relation to the gut microbiota.
Methods:Seventy GJs were sampled at four different regions, including two major Rabies hot spots, one minor hot spot and one Rabies free. Stool microbiota were analysed by 16S rRNA amplicon sequencing (Illumina MiSeq) and bioinformatics analysis was performed using the QIIME2 pipeline. We compared the GJ gut microbiota between geographic locations and different host traits and to available gut microbiota data of the Black-backed jackal (Canis mesomelas), a related African species.
Results:Gut microbiota analysis revealed 16 bacterial phyla but only 9 were >0.1% abundant (Figure 1). The most abundant families were Fusobacteriaceae (23%), Bacteroidetes (19.4%) and Prevotellaceae. Alpha diversity (Faith’s Phylogenetic Diversity) was found similar between geographic regions (p=0.15), and GJ sex and age groups (sex p=0.61; age class p=0.84). Beta diversity (Bray-Curtis distance) significantly differed between regions (p=0.001), and age groups (p=0.031), but not sex (p=0.5). Clustering of samples originating from rabies hot spots are shown in red (Figure 2). Firmicutes/Bacteroidetes ratio (high ratio is related to predicted immune dysfunction and obesity) was lower in the GJ (0.92±2.22) compared to its closest species, the Black-backed jackal (15.17±28.04).
Conclusions:Our study describes for the first time, the gut microbiota composition of the GJ. While alpha-diversity was similar between geographic location, sex and age, beta-diversity was significantly different between geographic location and age groups and notable differences in abundance were noted as compared to a related Jackal species. Better understanding of jackal-human interactions could underpin surveillance of golden jackal movements and assist in Rabies control efforts.
Session Type: ePosters
Session Title: ePosters
Authors(s): R. Lapid (1), Y. Motro (2), B. Khalfin (2), G. Kahila Bar-Gal (1), J. Moran-Gilad (2)
Authors Affiliations(s): (1) The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot 7610001, Israel, Israel, (2) Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel, Israel
Background:
The Golden Jackal (Canis aureus), is a medium canid carnivore which thrives in close proximity to human settlements, making it a potential reservoir for many zoonotic diseases. The GJ is one of the major reservoirs for rabies in Israel but has not been extensively studied. Despite growing research on animal microbiota, little is known regarding the GJ gut microbiota. Therefore, we sought to study the geographical spread of GJ across Rabies hot spots and investigate its relation to the gut microbiota.
Methods:Seventy GJs were sampled at four different regions, including two major Rabies hot spots, one minor hot spot and one Rabies free. Stool microbiota were analysed by 16S rRNA amplicon sequencing (Illumina MiSeq) and bioinformatics analysis was performed using the QIIME2 pipeline. We compared the GJ gut microbiota between geographic locations and different host traits and to available gut microbiota data of the Black-backed jackal (Canis mesomelas), a related African species.
Results:Gut microbiota analysis revealed 16 bacterial phyla but only 9 were >0.1% abundant (Figure 1). The most abundant families were Fusobacteriaceae (23%), Bacteroidetes (19.4%) and Prevotellaceae. Alpha diversity (Faith’s Phylogenetic Diversity) was found similar between geographic regions (p=0.15), and GJ sex and age groups (sex p=0.61; age class p=0.84). Beta diversity (Bray-Curtis distance) significantly differed between regions (p=0.001), and age groups (p=0.031), but not sex (p=0.5). Clustering of samples originating from rabies hot spots are shown in red (Figure 2). Firmicutes/Bacteroidetes ratio (high ratio is related to predicted immune dysfunction and obesity) was lower in the GJ (0.92±2.22) compared to its closest species, the Black-backed jackal (15.17±28.04).
Conclusions:Our study describes for the first time, the gut microbiota composition of the GJ. While alpha-diversity was similar between geographic location, sex and age, beta-diversity was significantly different between geographic location and age groups and notable differences in abundance were noted as compared to a related Jackal species. Better understanding of jackal-human interactions could underpin surveillance of golden jackal movements and assist in Rabies control efforts.
Session Type: ePosters
Session Title: ePosters
Authors(s): R. Lapid (1), Y. Motro (2), B. Khalfin (2), G. Kahila Bar-Gal (1), J. Moran-Gilad (2)
Authors Affiliations(s): (1) The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot 7610001, Israel, Israel, (2) Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel, Israel
Background:
The Golden Jackal (Canis aureus), is a medium canid carnivore which thrives in close proximity to human settlements, making it a potential reservoir for many zoonotic diseases. The GJ is one of the major reservoirs for rabies in Israel but has not been extensively studied. Despite growing research on animal microbiota, little is known regarding the GJ gut microbiota. Therefore, we sought to study the geographical spread of GJ across Rabies hot spots and investigate its relation to the gut microbiota.
Methods:Seventy GJs were sampled at four different regions, including two major Rabies hot spots, one minor hot spot and one Rabies free. Stool microbiota were analysed by 16S rRNA amplicon sequencing (Illumina MiSeq) and bioinformatics analysis was performed using the QIIME2 pipeline. We compared the GJ gut microbiota between geographic locations and different host traits and to available gut microbiota data of the Black-backed jackal (Canis mesomelas), a related African species.
Results:Gut microbiota analysis revealed 16 bacterial phyla but only 9 were >0.1% abundant (Figure 1). The most abundant families were Fusobacteriaceae (23%), Bacteroidetes (19.4%) and Prevotellaceae. Alpha diversity (Faith’s Phylogenetic Diversity) was found similar between geographic regions (p=0.15), and GJ sex and age groups (sex p=0.61; age class p=0.84). Beta diversity (Bray-Curtis distance) significantly differed between regions (p=0.001), and age groups (p=0.031), but not sex (p=0.5). Clustering of samples originating from rabies hot spots are shown in red (Figure 2). Firmicutes/Bacteroidetes ratio (high ratio is related to predicted immune dysfunction and obesity) was lower in the GJ (0.92±2.22) compared to its closest species, the Black-backed jackal (15.17±28.04).
Conclusions:Our study describes for the first time, the gut microbiota composition of the GJ. While alpha-diversity was similar between geographic location, sex and age, beta-diversity was significantly different between geographic location and age groups and notable differences in abundance were noted as compared to a related Jackal species. Better understanding of jackal-human interactions could underpin surveillance of golden jackal movements and assist in Rabies control efforts.
Session Type: ePosters
Session Title: ePosters
Authors(s): R. Lapid (1), Y. Motro (2), B. Khalfin (2), G. Kahila Bar-Gal (1), J. Moran-Gilad (2)
Authors Affiliations(s): (1) The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.B. 12, Rehovot 7610001, Israel, Israel, (2) Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel, Israel
Background:
The Golden Jackal (Canis aureus), is a medium canid carnivore which thrives in close proximity to human settlements, making it a potential reservoir for many zoonotic diseases. The GJ is one of the major reservoirs for rabies in Israel but has not been extensively studied. Despite growing research on animal microbiota, little is known regarding the GJ gut microbiota. Therefore, we sought to study the geographical spread of GJ across Rabies hot spots and investigate its relation to the gut microbiota.
Methods:Seventy GJs were sampled at four different regions, including two major Rabies hot spots, one minor hot spot and one Rabies free. Stool microbiota were analysed by 16S rRNA amplicon sequencing (Illumina MiSeq) and bioinformatics analysis was performed using the QIIME2 pipeline. We compared the GJ gut microbiota between geographic locations and different host traits and to available gut microbiota data of the Black-backed jackal (Canis mesomelas), a related African species.
Results:Gut microbiota analysis revealed 16 bacterial phyla but only 9 were >0.1% abundant (Figure 1). The most abundant families were Fusobacteriaceae (23%), Bacteroidetes (19.4%) and Prevotellaceae. Alpha diversity (Faith’s Phylogenetic Diversity) was found similar between geographic regions (p=0.15), and GJ sex and age groups (sex p=0.61; age class p=0.84). Beta diversity (Bray-Curtis distance) significantly differed between regions (p=0.001), and age groups (p=0.031), but not sex (p=0.5). Clustering of samples originating from rabies hot spots are shown in red (Figure 2). Firmicutes/Bacteroidetes ratio (high ratio is related to predicted immune dysfunction and obesity) was lower in the GJ (0.92±2.22) compared to its closest species, the Black-backed jackal (15.17±28.04).
Conclusions:Our study describes for the first time, the gut microbiota composition of the GJ. While alpha-diversity was similar between geographic location, sex and age, beta-diversity was significantly different between geographic location and age groups and notable differences in abundance were noted as compared to a related Jackal species. Better understanding of jackal-human interactions could underpin surveillance of golden jackal movements and assist in Rabies control efforts.