Session Type: ePosters
Session Title: ePosters
Authors(s): I. Bleriot (1), L. Blasco (1), O. Pacios (1), L. Fernandez-Garcia (1), A. Ambroa (1), M. Lopez (1), M. Gonzalez-Bardanca (1), F. Fernandez-Cuenca (2), J. Oteo (3), A. Pascual (2), L. Martinez-Martinez (4), P. Domingo-Calap (5), G. Bou (1), T. Wood (6), M. Tomas (1)
Authors Affiliations(s): (1) A Coruña Hospital (INIBIC), Spain, (2) Macarena Hospital (IBIS), Spain, (3) Institute of Health Carlos III, Spain, (4) University Hospital Reina Sofía, Spain, (5) Universitat de València, Spain, (6) Pennsylvania State University, United States
Third Party Affiliation: 85 publications (75 first quartile) in peer-reviewed journals indexed in Web of Science,> 10,199 citations, h-factor = 31; ii) Director in 3 defended doctoral theses; currently supervises 4 PhD students at INIBIC-CHUAC; iii) 7 Projects as Principal Investigator (PI) of which 3 research projects and 4 innovation projects and iv) 3 Patents, 2 international (PCT / EP2017 / 069849 and Argentina, 19083725) and a national patent (Spain, ES2449665) . iv) Evaluator of national and international projects. In addition, she is an associate editor for international journals with high impact factor. Finally, she participates as evaluator of national research projects (Call for Strategic Health Action-ISCIII) and European ones
Background:
Since their discovery, toxin-antitoxin(TA)systems have captivated the minds of many scientists, who attributed to them multiple functions in cell physiology.Recent studies revealed that the key role of TA systems could be phage-inhibition.In this context, the aim of this study was to investigate the role of the PemK-PemI type II TA system in the inhibition of 12 lytic bacteriophages.
Methods:Twelve lytic bacteriophages from wastewater were analyzed by TEM(Figure 1).In order to study the role of the PemK-PemI type II TA system in phage bacterial infection, we used these 12 lytic bacteriophages at a MOI of 0.1 in the isogenic model strain K. pneumoniae ATCC®10031TM(which lacks pemIK)containing pCA24N(empty plasmid),pCA24N(pemIK)(plasmid with cloned pemIK), or pCA24N(pemK)(plasmid with cloned pemK).Genes were expressed by IPTG induction.Next, the host-range of the phages was determined using the spot test technique with the collection of clinical strains of K. pneumoniae,which harbour the PemK-PemI type II TA system and the OXA48 carbapenemase.For comparison, native expression levels of PemK-PemI was tested by performing infection curves with bacteriophages vB_KpnP-VAC1(low infectivity)and vB_KpnS-VAC7(high infectivity)at a MOI of 0.1 in the indicated clinical strains:ST405-OXA48,ST16-OXA48 and ST974-OXA48 that contain the PemK-PemI TA system by plasmid.Finally,the relative expression of toxin and antitoxin genes during phage infection in the clinical strain ST16-OXA48 was studied by RT-qPCR.
Results:The results of the overexpression of the complete PemK-PemI TA system via pCA24N led to bacterial phage infection.However, overexpression of the pemK toxin alone from pCA24N prevented phage infection(Figures 1 and 2).Furthermore,the native expression of this TA system during vB_kpP-VAC1 and vB_KpS-VAC7 infection in the three clinical strains showed different phage bacterial infection patterns.Ultimately,the relative expression of the pemK toxin gene with respect to the pemI antitoxin gene in the clinical strain ST16-OXA48 after 15 min of infection revealed that the overexpression of the pemK toxin gene confers protection against phage-infection(Figure 3).
Conclusions:The results obtained throughout this study seem to indicate that it is the action of the PemK toxin,belonging to the PemK-PemI type II TA system,that protects the bacteria against phage infection.These findings requires further in-depth analysis to improve the understanding of the relationship between TA systems and bacterial phage infection.
Keyword(s): Klebsiella pneumoniae, Toxin-Antitoxin system, BacteriophagesSession Type: ePosters
Session Title: ePosters
Authors(s): I. Bleriot (1), L. Blasco (1), O. Pacios (1), L. Fernandez-Garcia (1), A. Ambroa (1), M. Lopez (1), M. Gonzalez-Bardanca (1), F. Fernandez-Cuenca (2), J. Oteo (3), A. Pascual (2), L. Martinez-Martinez (4), P. Domingo-Calap (5), G. Bou (1), T. Wood (6), M. Tomas (1)
Authors Affiliations(s): (1) A Coruña Hospital (INIBIC), Spain, (2) Macarena Hospital (IBIS), Spain, (3) Institute of Health Carlos III, Spain, (4) University Hospital Reina Sofía, Spain, (5) Universitat de València, Spain, (6) Pennsylvania State University, United States
Third Party Affiliation: 85 publications (75 first quartile) in peer-reviewed journals indexed in Web of Science,> 10,199 citations, h-factor = 31; ii) Director in 3 defended doctoral theses; currently supervises 4 PhD students at INIBIC-CHUAC; iii) 7 Projects as Principal Investigator (PI) of which 3 research projects and 4 innovation projects and iv) 3 Patents, 2 international (PCT / EP2017 / 069849 and Argentina, 19083725) and a national patent (Spain, ES2449665) . iv) Evaluator of national and international projects. In addition, she is an associate editor for international journals with high impact factor. Finally, she participates as evaluator of national research projects (Call for Strategic Health Action-ISCIII) and European ones
Background:
Since their discovery, toxin-antitoxin(TA)systems have captivated the minds of many scientists, who attributed to them multiple functions in cell physiology.Recent studies revealed that the key role of TA systems could be phage-inhibition.In this context, the aim of this study was to investigate the role of the PemK-PemI type II TA system in the inhibition of 12 lytic bacteriophages.
Methods:Twelve lytic bacteriophages from wastewater were analyzed by TEM(Figure 1).In order to study the role of the PemK-PemI type II TA system in phage bacterial infection, we used these 12 lytic bacteriophages at a MOI of 0.1 in the isogenic model strain K. pneumoniae ATCC®10031TM(which lacks pemIK)containing pCA24N(empty plasmid),pCA24N(pemIK)(plasmid with cloned pemIK), or pCA24N(pemK)(plasmid with cloned pemK).Genes were expressed by IPTG induction.Next, the host-range of the phages was determined using the spot test technique with the collection of clinical strains of K. pneumoniae,which harbour the PemK-PemI type II TA system and the OXA48 carbapenemase.For comparison, native expression levels of PemK-PemI was tested by performing infection curves with bacteriophages vB_KpnP-VAC1(low infectivity)and vB_KpnS-VAC7(high infectivity)at a MOI of 0.1 in the indicated clinical strains:ST405-OXA48,ST16-OXA48 and ST974-OXA48 that contain the PemK-PemI TA system by plasmid.Finally,the relative expression of toxin and antitoxin genes during phage infection in the clinical strain ST16-OXA48 was studied by RT-qPCR.
Results:The results of the overexpression of the complete PemK-PemI TA system via pCA24N led to bacterial phage infection.However, overexpression of the pemK toxin alone from pCA24N prevented phage infection(Figures 1 and 2).Furthermore,the native expression of this TA system during vB_kpP-VAC1 and vB_KpS-VAC7 infection in the three clinical strains showed different phage bacterial infection patterns.Ultimately,the relative expression of the pemK toxin gene with respect to the pemI antitoxin gene in the clinical strain ST16-OXA48 after 15 min of infection revealed that the overexpression of the pemK toxin gene confers protection against phage-infection(Figure 3).
Conclusions:The results obtained throughout this study seem to indicate that it is the action of the PemK toxin,belonging to the PemK-PemI type II TA system,that protects the bacteria against phage infection.These findings requires further in-depth analysis to improve the understanding of the relationship between TA systems and bacterial phage infection.
Keyword(s): Klebsiella pneumoniae, Toxin-Antitoxin system, Bacteriophages
Session Type: ePosters
Session Title: ePosters
Authors(s): I. Bleriot (1), L. Blasco (1), O. Pacios (1), L. Fernandez-Garcia (1), A. Ambroa (1), M. Lopez (1), M. Gonzalez-Bardanca (1), F. Fernandez-Cuenca (2), J. Oteo (3), A. Pascual (2), L. Martinez-Martinez (4), P. Domingo-Calap (5), G. Bou (1), T. Wood (6), M. Tomas (1)
Authors Affiliations(s): (1) A Coruña Hospital (INIBIC), Spain, (2) Macarena Hospital (IBIS), Spain, (3) Institute of Health Carlos III, Spain, (4) University Hospital Reina Sofía, Spain, (5) Universitat de València, Spain, (6) Pennsylvania State University, United States
Third Party Affiliation: 85 publications (75 first quartile) in peer-reviewed journals indexed in Web of Science,> 10,199 citations, h-factor = 31; ii) Director in 3 defended doctoral theses; currently supervises 4 PhD students at INIBIC-CHUAC; iii) 7 Projects as Principal Investigator (PI) of which 3 research projects and 4 innovation projects and iv) 3 Patents, 2 international (PCT / EP2017 / 069849 and Argentina, 19083725) and a national patent (Spain, ES2449665) . iv) Evaluator of national and international projects. In addition, she is an associate editor for international journals with high impact factor. Finally, she participates as evaluator of national research projects (Call for Strategic Health Action-ISCIII) and European ones
Background:
Since their discovery, toxin-antitoxin(TA)systems have captivated the minds of many scientists, who attributed to them multiple functions in cell physiology.Recent studies revealed that the key role of TA systems could be phage-inhibition.In this context, the aim of this study was to investigate the role of the PemK-PemI type II TA system in the inhibition of 12 lytic bacteriophages.
Methods:Twelve lytic bacteriophages from wastewater were analyzed by TEM(Figure 1).In order to study the role of the PemK-PemI type II TA system in phage bacterial infection, we used these 12 lytic bacteriophages at a MOI of 0.1 in the isogenic model strain K. pneumoniae ATCC®10031TM(which lacks pemIK)containing pCA24N(empty plasmid),pCA24N(pemIK)(plasmid with cloned pemIK), or pCA24N(pemK)(plasmid with cloned pemK).Genes were expressed by IPTG induction.Next, the host-range of the phages was determined using the spot test technique with the collection of clinical strains of K. pneumoniae,which harbour the PemK-PemI type II TA system and the OXA48 carbapenemase.For comparison, native expression levels of PemK-PemI was tested by performing infection curves with bacteriophages vB_KpnP-VAC1(low infectivity)and vB_KpnS-VAC7(high infectivity)at a MOI of 0.1 in the indicated clinical strains:ST405-OXA48,ST16-OXA48 and ST974-OXA48 that contain the PemK-PemI TA system by plasmid.Finally,the relative expression of toxin and antitoxin genes during phage infection in the clinical strain ST16-OXA48 was studied by RT-qPCR.
Results:The results of the overexpression of the complete PemK-PemI TA system via pCA24N led to bacterial phage infection.However, overexpression of the pemK toxin alone from pCA24N prevented phage infection(Figures 1 and 2).Furthermore,the native expression of this TA system during vB_kpP-VAC1 and vB_KpS-VAC7 infection in the three clinical strains showed different phage bacterial infection patterns.Ultimately,the relative expression of the pemK toxin gene with respect to the pemI antitoxin gene in the clinical strain ST16-OXA48 after 15 min of infection revealed that the overexpression of the pemK toxin gene confers protection against phage-infection(Figure 3).
Conclusions:The results obtained throughout this study seem to indicate that it is the action of the PemK toxin,belonging to the PemK-PemI type II TA system,that protects the bacteria against phage infection.These findings requires further in-depth analysis to improve the understanding of the relationship between TA systems and bacterial phage infection.
Keyword(s): Klebsiella pneumoniae, Toxin-Antitoxin system, BacteriophagesSession Type: ePosters
Session Title: ePosters
Authors(s): I. Bleriot (1), L. Blasco (1), O. Pacios (1), L. Fernandez-Garcia (1), A. Ambroa (1), M. Lopez (1), M. Gonzalez-Bardanca (1), F. Fernandez-Cuenca (2), J. Oteo (3), A. Pascual (2), L. Martinez-Martinez (4), P. Domingo-Calap (5), G. Bou (1), T. Wood (6), M. Tomas (1)
Authors Affiliations(s): (1) A Coruña Hospital (INIBIC), Spain, (2) Macarena Hospital (IBIS), Spain, (3) Institute of Health Carlos III, Spain, (4) University Hospital Reina Sofía, Spain, (5) Universitat de València, Spain, (6) Pennsylvania State University, United States
Third Party Affiliation: 85 publications (75 first quartile) in peer-reviewed journals indexed in Web of Science,> 10,199 citations, h-factor = 31; ii) Director in 3 defended doctoral theses; currently supervises 4 PhD students at INIBIC-CHUAC; iii) 7 Projects as Principal Investigator (PI) of which 3 research projects and 4 innovation projects and iv) 3 Patents, 2 international (PCT / EP2017 / 069849 and Argentina, 19083725) and a national patent (Spain, ES2449665) . iv) Evaluator of national and international projects. In addition, she is an associate editor for international journals with high impact factor. Finally, she participates as evaluator of national research projects (Call for Strategic Health Action-ISCIII) and European ones
Background:
Since their discovery, toxin-antitoxin(TA)systems have captivated the minds of many scientists, who attributed to them multiple functions in cell physiology.Recent studies revealed that the key role of TA systems could be phage-inhibition.In this context, the aim of this study was to investigate the role of the PemK-PemI type II TA system in the inhibition of 12 lytic bacteriophages.
Methods:Twelve lytic bacteriophages from wastewater were analyzed by TEM(Figure 1).In order to study the role of the PemK-PemI type II TA system in phage bacterial infection, we used these 12 lytic bacteriophages at a MOI of 0.1 in the isogenic model strain K. pneumoniae ATCC®10031TM(which lacks pemIK)containing pCA24N(empty plasmid),pCA24N(pemIK)(plasmid with cloned pemIK), or pCA24N(pemK)(plasmid with cloned pemK).Genes were expressed by IPTG induction.Next, the host-range of the phages was determined using the spot test technique with the collection of clinical strains of K. pneumoniae,which harbour the PemK-PemI type II TA system and the OXA48 carbapenemase.For comparison, native expression levels of PemK-PemI was tested by performing infection curves with bacteriophages vB_KpnP-VAC1(low infectivity)and vB_KpnS-VAC7(high infectivity)at a MOI of 0.1 in the indicated clinical strains:ST405-OXA48,ST16-OXA48 and ST974-OXA48 that contain the PemK-PemI TA system by plasmid.Finally,the relative expression of toxin and antitoxin genes during phage infection in the clinical strain ST16-OXA48 was studied by RT-qPCR.
Results:The results of the overexpression of the complete PemK-PemI TA system via pCA24N led to bacterial phage infection.However, overexpression of the pemK toxin alone from pCA24N prevented phage infection(Figures 1 and 2).Furthermore,the native expression of this TA system during vB_kpP-VAC1 and vB_KpS-VAC7 infection in the three clinical strains showed different phage bacterial infection patterns.Ultimately,the relative expression of the pemK toxin gene with respect to the pemI antitoxin gene in the clinical strain ST16-OXA48 after 15 min of infection revealed that the overexpression of the pemK toxin gene confers protection against phage-infection(Figure 3).
Conclusions:The results obtained throughout this study seem to indicate that it is the action of the PemK toxin,belonging to the PemK-PemI type II TA system,that protects the bacteria against phage infection.These findings requires further in-depth analysis to improve the understanding of the relationship between TA systems and bacterial phage infection.
Keyword(s): Klebsiella pneumoniae, Toxin-Antitoxin system, Bacteriophages