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Abstract
Discussion Forum (0)
Abstract number: 818

Session Type: ePosters

Session Title: ePosters

Authors(s): H. Yang, B. Gu

Authors Affiliations(s): Xuzhou Medical University, China

Background:

Background: In developing countries, Shigella is the main force in childhood morbidity, death and stunting. The emergence of antibiotic resistance in Shigella necessitates the exploration of new treatment.The gut microbiota and its metabolite can affect the host's health, particularly with regard to intestinal inflammation. Indole-3-carboxaldehyde(I3A)is a biologically active metabolite produced by the gut commensal microbiota. However, the role of I3A in Shigella-induced colitis has not yet been clarified. Here, we targeted to explore the protective effect of I3A in a mouse model of colitis caused by Shigella infection and to clarify the underlying mechanism.

Methods:

Methods: Firstly, we detected the minimum inhibitory concentration (MIC) of I3A on Shigella, and then the OD600 and coated plate count were used to detect the effect of I3A on Shigella viability. Then Shigella infection model in mice were established with I3A treatment and observed the mouse body weight and disease activity index (DAI) every day. After the mice were sacrificed, we compared the length of the colon, observed the damage of the colonic mucosal tissue by hematoxylin-eosin (HE) staining, detected the amount of bacteria in the faeces and caecum content, and used the Quantitative Real-time PCR (qRT-PCR) to quantitatively analyze inflammatory cytokines.

Results:

Results: The MIC of I3A was 128 ug/mL, and both the OD600 and the coated plate proved that I3A could effectively inhibit Shigella. In addition, I3A reduced DAI and prevented colon shortening and improved the mucus barrier in Shigella-induced colitis.I3A also reduces the levels of pro-inflammatory cytokines and induces the increase of anti-inflammatory factors,thereby playing a protective role in Shigella-induced colitis.

Conclusions:

Conclusion: These studies showed that I3A can inhibit Shigella, regulate the immune response and improve the intestinal mucus barrier, thereby playing a protective role in Shigella infectious colitis. Our research provides a broad prospect for exploring gut microbes which can improve colitis induced by Shigella.

Keyword(s): indole-3-carboxaldehydegut microbiota metabolites, gut microbiota metabolites, Shigella

Abstract number: 818

Session Type: ePosters

Session Title: ePosters

Authors(s): H. Yang, B. Gu

Authors Affiliations(s): Xuzhou Medical University, China

Background:

Background: In developing countries, Shigella is the main force in childhood morbidity, death and stunting. The emergence of antibiotic resistance in Shigella necessitates the exploration of new treatment.The gut microbiota and its metabolite can affect the host's health, particularly with regard to intestinal inflammation. Indole-3-carboxaldehyde(I3A)is a biologically active metabolite produced by the gut commensal microbiota. However, the role of I3A in Shigella-induced colitis has not yet been clarified. Here, we targeted to explore the protective effect of I3A in a mouse model of colitis caused by Shigella infection and to clarify the underlying mechanism.

Methods:

Methods: Firstly, we detected the minimum inhibitory concentration (MIC) of I3A on Shigella, and then the OD600 and coated plate count were used to detect the effect of I3A on Shigella viability. Then Shigella infection model in mice were established with I3A treatment and observed the mouse body weight and disease activity index (DAI) every day. After the mice were sacrificed, we compared the length of the colon, observed the damage of the colonic mucosal tissue by hematoxylin-eosin (HE) staining, detected the amount of bacteria in the faeces and caecum content, and used the Quantitative Real-time PCR (qRT-PCR) to quantitatively analyze inflammatory cytokines.

Results:

Results: The MIC of I3A was 128 ug/mL, and both the OD600 and the coated plate proved that I3A could effectively inhibit Shigella. In addition, I3A reduced DAI and prevented colon shortening and improved the mucus barrier in Shigella-induced colitis.I3A also reduces the levels of pro-inflammatory cytokines and induces the increase of anti-inflammatory factors,thereby playing a protective role in Shigella-induced colitis.

Conclusions:

Conclusion: These studies showed that I3A can inhibit Shigella, regulate the immune response and improve the intestinal mucus barrier, thereby playing a protective role in Shigella infectious colitis. Our research provides a broad prospect for exploring gut microbes which can improve colitis induced by Shigella.

Keyword(s): indole-3-carboxaldehydegut microbiota metabolites, gut microbiota metabolites, Shigella

I3A ameliorates Shigella infectious colitis by inhibiting Shigella and regulating the immune response
Huan Yang
Huan Yang
ESCMID eAcademy. Yang H. 07/09/2021; 327813; 818;
user
Huan Yang
Abstract
Discussion Forum (0)
Abstract number: 818

Session Type: ePosters

Session Title: ePosters

Authors(s): H. Yang, B. Gu

Authors Affiliations(s): Xuzhou Medical University, China

Background:

Background: In developing countries, Shigella is the main force in childhood morbidity, death and stunting. The emergence of antibiotic resistance in Shigella necessitates the exploration of new treatment.The gut microbiota and its metabolite can affect the host's health, particularly with regard to intestinal inflammation. Indole-3-carboxaldehyde(I3A)is a biologically active metabolite produced by the gut commensal microbiota. However, the role of I3A in Shigella-induced colitis has not yet been clarified. Here, we targeted to explore the protective effect of I3A in a mouse model of colitis caused by Shigella infection and to clarify the underlying mechanism.

Methods:

Methods: Firstly, we detected the minimum inhibitory concentration (MIC) of I3A on Shigella, and then the OD600 and coated plate count were used to detect the effect of I3A on Shigella viability. Then Shigella infection model in mice were established with I3A treatment and observed the mouse body weight and disease activity index (DAI) every day. After the mice were sacrificed, we compared the length of the colon, observed the damage of the colonic mucosal tissue by hematoxylin-eosin (HE) staining, detected the amount of bacteria in the faeces and caecum content, and used the Quantitative Real-time PCR (qRT-PCR) to quantitatively analyze inflammatory cytokines.

Results:

Results: The MIC of I3A was 128 ug/mL, and both the OD600 and the coated plate proved that I3A could effectively inhibit Shigella. In addition, I3A reduced DAI and prevented colon shortening and improved the mucus barrier in Shigella-induced colitis.I3A also reduces the levels of pro-inflammatory cytokines and induces the increase of anti-inflammatory factors,thereby playing a protective role in Shigella-induced colitis.

Conclusions:

Conclusion: These studies showed that I3A can inhibit Shigella, regulate the immune response and improve the intestinal mucus barrier, thereby playing a protective role in Shigella infectious colitis. Our research provides a broad prospect for exploring gut microbes which can improve colitis induced by Shigella.

Keyword(s): indole-3-carboxaldehydegut microbiota metabolites, gut microbiota metabolites, Shigella

Abstract number: 818

Session Type: ePosters

Session Title: ePosters

Authors(s): H. Yang, B. Gu

Authors Affiliations(s): Xuzhou Medical University, China

Background:

Background: In developing countries, Shigella is the main force in childhood morbidity, death and stunting. The emergence of antibiotic resistance in Shigella necessitates the exploration of new treatment.The gut microbiota and its metabolite can affect the host's health, particularly with regard to intestinal inflammation. Indole-3-carboxaldehyde(I3A)is a biologically active metabolite produced by the gut commensal microbiota. However, the role of I3A in Shigella-induced colitis has not yet been clarified. Here, we targeted to explore the protective effect of I3A in a mouse model of colitis caused by Shigella infection and to clarify the underlying mechanism.

Methods:

Methods: Firstly, we detected the minimum inhibitory concentration (MIC) of I3A on Shigella, and then the OD600 and coated plate count were used to detect the effect of I3A on Shigella viability. Then Shigella infection model in mice were established with I3A treatment and observed the mouse body weight and disease activity index (DAI) every day. After the mice were sacrificed, we compared the length of the colon, observed the damage of the colonic mucosal tissue by hematoxylin-eosin (HE) staining, detected the amount of bacteria in the faeces and caecum content, and used the Quantitative Real-time PCR (qRT-PCR) to quantitatively analyze inflammatory cytokines.

Results:

Results: The MIC of I3A was 128 ug/mL, and both the OD600 and the coated plate proved that I3A could effectively inhibit Shigella. In addition, I3A reduced DAI and prevented colon shortening and improved the mucus barrier in Shigella-induced colitis.I3A also reduces the levels of pro-inflammatory cytokines and induces the increase of anti-inflammatory factors,thereby playing a protective role in Shigella-induced colitis.

Conclusions:

Conclusion: These studies showed that I3A can inhibit Shigella, regulate the immune response and improve the intestinal mucus barrier, thereby playing a protective role in Shigella infectious colitis. Our research provides a broad prospect for exploring gut microbes which can improve colitis induced by Shigella.

Keyword(s): indole-3-carboxaldehydegut microbiota metabolites, gut microbiota metabolites, Shigella

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