Session Type: ePosters
Session Title: ePosters
Authors(s): S. Mukherjee (1, 2), S. Naha (1), P. Bhadury (3), B. Saha (4), S. Dutta (1), S. Basu (1)
Authors Affiliations(s): (1) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases. - Kolkata (India), India, (2) Department of Zoology, Government General Degree College, Singur. - Hooghly (India), India, (3) Integrative Taxonomy and Microbial Ecology Research Group, Department of Biological Sciences, Indian Institute of Science Education and Research - Kolkata (India), India, (4) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases., India
Background:
Majority of hospital-acquired infections reported globally are due to the classical K. pneumoniae. Lately, the emergence of hypervirulent K. pneumoniae (hvKP) have posed a greater challenge to clinicians. These hvKPs have now acquired carbapenem resistance determinants (CR-hvKP) leading to a dearth of therapeutic options. CR-hvKP strains causing neonatal sepsis were characterized in terms of virulence and carbapenem resistance.
Methods:One hundred seven K. pneumoniae were isolated from septicemic neonates, of which nine non-duplicate CR-hvKP strains were detected. Virulence potential was evaluated on the basis of PCRs, biofilm and serum resistance assay. Carbapenem resistance was assessed by antimicrobial susceptibility tests, carbapenem-resistant determinants and conjugation. Sequence typing, capsular typing and whole genome sequencing (WGS) was performed.
Results:All nine isolates were established as hypervirulent on the basis of the hvKP-associated capsular types (K2, K20, and K54) and belonged to six different sequence types (ST11, ST14, ST15, ST65, ST268, and ST307). Among the nine CR-hvKP, seven isolates harboured blaNDM-1 and two harboured blaNDM-5, of them one strain co-harboured blaOXA-232. All the transconjugants exhibited MICs for carbapenems, ranging between 2-32 mg/L. blaNDM was associated with several plasmid-replicon types (IncA/C, IncR, IncFII, IncFIIK, and IncHIB-M/FIB-M). The CR-hvKPs also harboured wabG, uge, wcaJ, fimH, mrkD, entB, and ybtS virulence genes. WGS was done for two NDM-1-producing CR-hvKPs possessing pLVPK-associated hypervirulent markers. WGS revealed the genome of EN5180 (ST15, K54) and EN5289 (ST11, K2) harboured 96 and 86 putative virulence factors, respectively, including pLVPK-associated markers (rmpA, rmpA2, iroBCDEN and iucABCD, iutA . Moreover, comparative genomic analysis with the other hvKP reference genomes showed ~94% of inter-genomic resemblance. Most CR-hvKPs exhibited strong biofilm-forming capability and were also resistant against normal human serum.
The pLVPK-associated markers were co-transferred along with blaNDM-1 in the same IncHIB-M/FIB-M plasmid strongly suggesting the association of both highly transmissible AMR and hypervirulent determinants in the same conjugative plasmid.
Conclusions:To the best of our knowledge, this is the first report of NDM-1-producing ST11 and ST15 strains causing neonatal sepsis. In neonates, the combined effect of an immature immune system and the involvement of drug-resistant hypervirulent CR-hvKP is worrisome.
Keyword(s): Hypervirulence, Neonatal sepsis, Carbapenem resistanceCOI Other: No other support is available as of date. The presenting author would apply for the Programme Book Grant Initiative 2021.
Session Type: ePosters
Session Title: ePosters
Authors(s): S. Mukherjee (1, 2), S. Naha (1), P. Bhadury (3), B. Saha (4), S. Dutta (1), S. Basu (1)
Authors Affiliations(s): (1) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases. - Kolkata (India), India, (2) Department of Zoology, Government General Degree College, Singur. - Hooghly (India), India, (3) Integrative Taxonomy and Microbial Ecology Research Group, Department of Biological Sciences, Indian Institute of Science Education and Research - Kolkata (India), India, (4) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases., India
Background:
Majority of hospital-acquired infections reported globally are due to the classical K. pneumoniae. Lately, the emergence of hypervirulent K. pneumoniae (hvKP) have posed a greater challenge to clinicians. These hvKPs have now acquired carbapenem resistance determinants (CR-hvKP) leading to a dearth of therapeutic options. CR-hvKP strains causing neonatal sepsis were characterized in terms of virulence and carbapenem resistance.
Methods:One hundred seven K. pneumoniae were isolated from septicemic neonates, of which nine non-duplicate CR-hvKP strains were detected. Virulence potential was evaluated on the basis of PCRs, biofilm and serum resistance assay. Carbapenem resistance was assessed by antimicrobial susceptibility tests, carbapenem-resistant determinants and conjugation. Sequence typing, capsular typing and whole genome sequencing (WGS) was performed.
Results:All nine isolates were established as hypervirulent on the basis of the hvKP-associated capsular types (K2, K20, and K54) and belonged to six different sequence types (ST11, ST14, ST15, ST65, ST268, and ST307). Among the nine CR-hvKP, seven isolates harboured blaNDM-1 and two harboured blaNDM-5, of them one strain co-harboured blaOXA-232. All the transconjugants exhibited MICs for carbapenems, ranging between 2-32 mg/L. blaNDM was associated with several plasmid-replicon types (IncA/C, IncR, IncFII, IncFIIK, and IncHIB-M/FIB-M). The CR-hvKPs also harboured wabG, uge, wcaJ, fimH, mrkD, entB, and ybtS virulence genes. WGS was done for two NDM-1-producing CR-hvKPs possessing pLVPK-associated hypervirulent markers. WGS revealed the genome of EN5180 (ST15, K54) and EN5289 (ST11, K2) harboured 96 and 86 putative virulence factors, respectively, including pLVPK-associated markers (rmpA, rmpA2, iroBCDEN and iucABCD, iutA . Moreover, comparative genomic analysis with the other hvKP reference genomes showed ~94% of inter-genomic resemblance. Most CR-hvKPs exhibited strong biofilm-forming capability and were also resistant against normal human serum.
The pLVPK-associated markers were co-transferred along with blaNDM-1 in the same IncHIB-M/FIB-M plasmid strongly suggesting the association of both highly transmissible AMR and hypervirulent determinants in the same conjugative plasmid.
Conclusions:To the best of our knowledge, this is the first report of NDM-1-producing ST11 and ST15 strains causing neonatal sepsis. In neonates, the combined effect of an immature immune system and the involvement of drug-resistant hypervirulent CR-hvKP is worrisome.
Keyword(s): Hypervirulence, Neonatal sepsis, Carbapenem resistanceCOI Other: No other support is available as of date. The presenting author would apply for the Programme Book Grant Initiative 2021.
Session Type: ePosters
Session Title: ePosters
Authors(s): S. Mukherjee (1, 2), S. Naha (1), P. Bhadury (3), B. Saha (4), S. Dutta (1), S. Basu (1)
Authors Affiliations(s): (1) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases. - Kolkata (India), India, (2) Department of Zoology, Government General Degree College, Singur. - Hooghly (India), India, (3) Integrative Taxonomy and Microbial Ecology Research Group, Department of Biological Sciences, Indian Institute of Science Education and Research - Kolkata (India), India, (4) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases., India
Background:
Majority of hospital-acquired infections reported globally are due to the classical K. pneumoniae. Lately, the emergence of hypervirulent K. pneumoniae (hvKP) have posed a greater challenge to clinicians. These hvKPs have now acquired carbapenem resistance determinants (CR-hvKP) leading to a dearth of therapeutic options. CR-hvKP strains causing neonatal sepsis were characterized in terms of virulence and carbapenem resistance.
Methods:One hundred seven K. pneumoniae were isolated from septicemic neonates, of which nine non-duplicate CR-hvKP strains were detected. Virulence potential was evaluated on the basis of PCRs, biofilm and serum resistance assay. Carbapenem resistance was assessed by antimicrobial susceptibility tests, carbapenem-resistant determinants and conjugation. Sequence typing, capsular typing and whole genome sequencing (WGS) was performed.
Results:All nine isolates were established as hypervirulent on the basis of the hvKP-associated capsular types (K2, K20, and K54) and belonged to six different sequence types (ST11, ST14, ST15, ST65, ST268, and ST307). Among the nine CR-hvKP, seven isolates harboured blaNDM-1 and two harboured blaNDM-5, of them one strain co-harboured blaOXA-232. All the transconjugants exhibited MICs for carbapenems, ranging between 2-32 mg/L. blaNDM was associated with several plasmid-replicon types (IncA/C, IncR, IncFII, IncFIIK, and IncHIB-M/FIB-M). The CR-hvKPs also harboured wabG, uge, wcaJ, fimH, mrkD, entB, and ybtS virulence genes. WGS was done for two NDM-1-producing CR-hvKPs possessing pLVPK-associated hypervirulent markers. WGS revealed the genome of EN5180 (ST15, K54) and EN5289 (ST11, K2) harboured 96 and 86 putative virulence factors, respectively, including pLVPK-associated markers (rmpA, rmpA2, iroBCDEN and iucABCD, iutA . Moreover, comparative genomic analysis with the other hvKP reference genomes showed ~94% of inter-genomic resemblance. Most CR-hvKPs exhibited strong biofilm-forming capability and were also resistant against normal human serum.
The pLVPK-associated markers were co-transferred along with blaNDM-1 in the same IncHIB-M/FIB-M plasmid strongly suggesting the association of both highly transmissible AMR and hypervirulent determinants in the same conjugative plasmid.
Conclusions:To the best of our knowledge, this is the first report of NDM-1-producing ST11 and ST15 strains causing neonatal sepsis. In neonates, the combined effect of an immature immune system and the involvement of drug-resistant hypervirulent CR-hvKP is worrisome.
Keyword(s): Hypervirulence, Neonatal sepsis, Carbapenem resistanceCOI Other: No other support is available as of date. The presenting author would apply for the Programme Book Grant Initiative 2021.
Session Type: ePosters
Session Title: ePosters
Authors(s): S. Mukherjee (1, 2), S. Naha (1), P. Bhadury (3), B. Saha (4), S. Dutta (1), S. Basu (1)
Authors Affiliations(s): (1) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases. - Kolkata (India), India, (2) Department of Zoology, Government General Degree College, Singur. - Hooghly (India), India, (3) Integrative Taxonomy and Microbial Ecology Research Group, Department of Biological Sciences, Indian Institute of Science Education and Research - Kolkata (India), India, (4) Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases., India
Background:
Majority of hospital-acquired infections reported globally are due to the classical K. pneumoniae. Lately, the emergence of hypervirulent K. pneumoniae (hvKP) have posed a greater challenge to clinicians. These hvKPs have now acquired carbapenem resistance determinants (CR-hvKP) leading to a dearth of therapeutic options. CR-hvKP strains causing neonatal sepsis were characterized in terms of virulence and carbapenem resistance.
Methods:One hundred seven K. pneumoniae were isolated from septicemic neonates, of which nine non-duplicate CR-hvKP strains were detected. Virulence potential was evaluated on the basis of PCRs, biofilm and serum resistance assay. Carbapenem resistance was assessed by antimicrobial susceptibility tests, carbapenem-resistant determinants and conjugation. Sequence typing, capsular typing and whole genome sequencing (WGS) was performed.
Results:All nine isolates were established as hypervirulent on the basis of the hvKP-associated capsular types (K2, K20, and K54) and belonged to six different sequence types (ST11, ST14, ST15, ST65, ST268, and ST307). Among the nine CR-hvKP, seven isolates harboured blaNDM-1 and two harboured blaNDM-5, of them one strain co-harboured blaOXA-232. All the transconjugants exhibited MICs for carbapenems, ranging between 2-32 mg/L. blaNDM was associated with several plasmid-replicon types (IncA/C, IncR, IncFII, IncFIIK, and IncHIB-M/FIB-M). The CR-hvKPs also harboured wabG, uge, wcaJ, fimH, mrkD, entB, and ybtS virulence genes. WGS was done for two NDM-1-producing CR-hvKPs possessing pLVPK-associated hypervirulent markers. WGS revealed the genome of EN5180 (ST15, K54) and EN5289 (ST11, K2) harboured 96 and 86 putative virulence factors, respectively, including pLVPK-associated markers (rmpA, rmpA2, iroBCDEN and iucABCD, iutA . Moreover, comparative genomic analysis with the other hvKP reference genomes showed ~94% of inter-genomic resemblance. Most CR-hvKPs exhibited strong biofilm-forming capability and were also resistant against normal human serum.
The pLVPK-associated markers were co-transferred along with blaNDM-1 in the same IncHIB-M/FIB-M plasmid strongly suggesting the association of both highly transmissible AMR and hypervirulent determinants in the same conjugative plasmid.
Conclusions:To the best of our knowledge, this is the first report of NDM-1-producing ST11 and ST15 strains causing neonatal sepsis. In neonates, the combined effect of an immature immune system and the involvement of drug-resistant hypervirulent CR-hvKP is worrisome.
Keyword(s): Hypervirulence, Neonatal sepsis, Carbapenem resistanceCOI Other: No other support is available as of date. The presenting author would apply for the Programme Book Grant Initiative 2021.