Session Type: ePosters
Session Title: ePosters
Authors(s): M. Ercibengoa, M. Alonso, N. Azcue, P. Vallejo, A. Sorarrain, M. Gomez-Ruiz De Arbulo, D. Vicente, G. Cilla, J.M. Marimon
Authors Affiliations(s): Biodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group; Osakidetza Basque Health Service, Donostialdea Integrated Health Organisation, Microbiology Department, 20014 San Sebastian, Spain, Spain
Background:
Pediatric nocardiosis is a rare entity being, associated with immunodeficiency processes such as chronic granulomatous disease, organ transplants and treatment with steroids. Due to its infrequency, the knowledge of pediatric nocardiosis is minimal and somehow outdated. The aim of this work was to describe the incidence of nocardiosis in children in our area.
Case:Retrospective 22 -year study that included all Nocardia isolates collected at Donostia University Hospital (San Sebastian, Basque Country, northern Spain) between January 1998 and December 2020. The hospital has 1100- bed, serves a general population of 716,834 inhabitants (101,013 children under 15 years) and is the referral hospital for the province. Nocardia isolates were identified at species level by sequencing 16srRNA, gyrB, hsp 65, rpoB, and secA1 genes. Sequences were compared with sequences of other Nocardia isolates available at GenBank www.ncbi.nlm.nih.gov/genbank/) and in leBIBI (www.pbil.univ-lyon1.fr/bibi) databases. Antimicrobial susceptibility testing of Nocardia isolates was performed by the broth microdilution method according to CLSI guidelines.
Overall, 342 non-duplicated isolates were obtained. Of them, only 2 (0.6%) were recovered from children < 15 years being both in two immunocompetent patients. Both were primary cutaneous nocardiosis representing 40% of total cutaneous nocardiosis detected. In the first case, a N. farcinica was isolated from a node placed 2 cm away where a 4 months old female had received BCG vaccination. In the second case, a N. brasiliensis was isolated from the elbow of a 9-year old boy who had had an episode of itchy and papulous dermatitis with lesions on the elbows, neck, ankles and torso two months before the onset of the symptoms. Both N. brasiliensis and N. farcinica were susceptible to amoxicillin-clavulanate (MIC = 0.5/9.5 and trimethoprim-sulfamethoxazole) (MIC = 0.5/9.5 µg/ml). N. brasiliensis, showed non-susceptibility to imipenem (MIC = 64µg/ml), ciprofloxacin (MIC >4 µg/ml), linezolid (MIC = 2 µg/ml). N. farcinica was non-susceptible to amoxicillin/ampicillin (MIC = 64µg/ml), clindamycin (MIC >4 µg/ml) and vancomycin (MIC >4 µg/ml).
Discussion:In Gipuzkoa (Basque Country, Spain) pediatric nocardiosis is a rare entity. Two cases, of primary cutaneous nocardiosis were diagnosed in two immunocompetent children, representing <1% of total nocardiosis detected.
Keyword(s): Nocardiosis cutánea, niños menores de 15 años, Nocardia farcinica, Nocardia brasiliensisCOI Other: No conflict of interest
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Ercibengoa, M. Alonso, N. Azcue, P. Vallejo, A. Sorarrain, M. Gomez-Ruiz De Arbulo, D. Vicente, G. Cilla, J.M. Marimon
Authors Affiliations(s): Biodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group; Osakidetza Basque Health Service, Donostialdea Integrated Health Organisation, Microbiology Department, 20014 San Sebastian, Spain, Spain
Background:
Pediatric nocardiosis is a rare entity being, associated with immunodeficiency processes such as chronic granulomatous disease, organ transplants and treatment with steroids. Due to its infrequency, the knowledge of pediatric nocardiosis is minimal and somehow outdated. The aim of this work was to describe the incidence of nocardiosis in children in our area.
Case:Retrospective 22 -year study that included all Nocardia isolates collected at Donostia University Hospital (San Sebastian, Basque Country, northern Spain) between January 1998 and December 2020. The hospital has 1100- bed, serves a general population of 716,834 inhabitants (101,013 children under 15 years) and is the referral hospital for the province. Nocardia isolates were identified at species level by sequencing 16srRNA, gyrB, hsp 65, rpoB, and secA1 genes. Sequences were compared with sequences of other Nocardia isolates available at GenBank www.ncbi.nlm.nih.gov/genbank/) and in leBIBI (www.pbil.univ-lyon1.fr/bibi) databases. Antimicrobial susceptibility testing of Nocardia isolates was performed by the broth microdilution method according to CLSI guidelines.
Overall, 342 non-duplicated isolates were obtained. Of them, only 2 (0.6%) were recovered from children < 15 years being both in two immunocompetent patients. Both were primary cutaneous nocardiosis representing 40% of total cutaneous nocardiosis detected. In the first case, a N. farcinica was isolated from a node placed 2 cm away where a 4 months old female had received BCG vaccination. In the second case, a N. brasiliensis was isolated from the elbow of a 9-year old boy who had had an episode of itchy and papulous dermatitis with lesions on the elbows, neck, ankles and torso two months before the onset of the symptoms. Both N. brasiliensis and N. farcinica were susceptible to amoxicillin-clavulanate (MIC = 0.5/9.5 and trimethoprim-sulfamethoxazole) (MIC = 0.5/9.5 µg/ml). N. brasiliensis, showed non-susceptibility to imipenem (MIC = 64µg/ml), ciprofloxacin (MIC >4 µg/ml), linezolid (MIC = 2 µg/ml). N. farcinica was non-susceptible to amoxicillin/ampicillin (MIC = 64µg/ml), clindamycin (MIC >4 µg/ml) and vancomycin (MIC >4 µg/ml).
Discussion:In Gipuzkoa (Basque Country, Spain) pediatric nocardiosis is a rare entity. Two cases, of primary cutaneous nocardiosis were diagnosed in two immunocompetent children, representing <1% of total nocardiosis detected.
Keyword(s): Nocardiosis cutánea, niños menores de 15 años, Nocardia farcinica, Nocardia brasiliensisCOI Other: No conflict of interest
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Ercibengoa, M. Alonso, N. Azcue, P. Vallejo, A. Sorarrain, M. Gomez-Ruiz De Arbulo, D. Vicente, G. Cilla, J.M. Marimon
Authors Affiliations(s): Biodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group; Osakidetza Basque Health Service, Donostialdea Integrated Health Organisation, Microbiology Department, 20014 San Sebastian, Spain, Spain
Background:
Pediatric nocardiosis is a rare entity being, associated with immunodeficiency processes such as chronic granulomatous disease, organ transplants and treatment with steroids. Due to its infrequency, the knowledge of pediatric nocardiosis is minimal and somehow outdated. The aim of this work was to describe the incidence of nocardiosis in children in our area.
Case:Retrospective 22 -year study that included all Nocardia isolates collected at Donostia University Hospital (San Sebastian, Basque Country, northern Spain) between January 1998 and December 2020. The hospital has 1100- bed, serves a general population of 716,834 inhabitants (101,013 children under 15 years) and is the referral hospital for the province. Nocardia isolates were identified at species level by sequencing 16srRNA, gyrB, hsp 65, rpoB, and secA1 genes. Sequences were compared with sequences of other Nocardia isolates available at GenBank www.ncbi.nlm.nih.gov/genbank/) and in leBIBI (www.pbil.univ-lyon1.fr/bibi) databases. Antimicrobial susceptibility testing of Nocardia isolates was performed by the broth microdilution method according to CLSI guidelines.
Overall, 342 non-duplicated isolates were obtained. Of them, only 2 (0.6%) were recovered from children < 15 years being both in two immunocompetent patients. Both were primary cutaneous nocardiosis representing 40% of total cutaneous nocardiosis detected. In the first case, a N. farcinica was isolated from a node placed 2 cm away where a 4 months old female had received BCG vaccination. In the second case, a N. brasiliensis was isolated from the elbow of a 9-year old boy who had had an episode of itchy and papulous dermatitis with lesions on the elbows, neck, ankles and torso two months before the onset of the symptoms. Both N. brasiliensis and N. farcinica were susceptible to amoxicillin-clavulanate (MIC = 0.5/9.5 and trimethoprim-sulfamethoxazole) (MIC = 0.5/9.5 µg/ml). N. brasiliensis, showed non-susceptibility to imipenem (MIC = 64µg/ml), ciprofloxacin (MIC >4 µg/ml), linezolid (MIC = 2 µg/ml). N. farcinica was non-susceptible to amoxicillin/ampicillin (MIC = 64µg/ml), clindamycin (MIC >4 µg/ml) and vancomycin (MIC >4 µg/ml).
Discussion:In Gipuzkoa (Basque Country, Spain) pediatric nocardiosis is a rare entity. Two cases, of primary cutaneous nocardiosis were diagnosed in two immunocompetent children, representing <1% of total nocardiosis detected.
Keyword(s): Nocardiosis cutánea, niños menores de 15 años, Nocardia farcinica, Nocardia brasiliensisCOI Other: No conflict of interest
Session Type: ePosters
Session Title: ePosters
Authors(s): M. Ercibengoa, M. Alonso, N. Azcue, P. Vallejo, A. Sorarrain, M. Gomez-Ruiz De Arbulo, D. Vicente, G. Cilla, J.M. Marimon
Authors Affiliations(s): Biodonostia, Infectious Diseases Area, Respiratory Infection and Antimicrobial Resistance Group; Osakidetza Basque Health Service, Donostialdea Integrated Health Organisation, Microbiology Department, 20014 San Sebastian, Spain, Spain
Background:
Pediatric nocardiosis is a rare entity being, associated with immunodeficiency processes such as chronic granulomatous disease, organ transplants and treatment with steroids. Due to its infrequency, the knowledge of pediatric nocardiosis is minimal and somehow outdated. The aim of this work was to describe the incidence of nocardiosis in children in our area.
Case:Retrospective 22 -year study that included all Nocardia isolates collected at Donostia University Hospital (San Sebastian, Basque Country, northern Spain) between January 1998 and December 2020. The hospital has 1100- bed, serves a general population of 716,834 inhabitants (101,013 children under 15 years) and is the referral hospital for the province. Nocardia isolates were identified at species level by sequencing 16srRNA, gyrB, hsp 65, rpoB, and secA1 genes. Sequences were compared with sequences of other Nocardia isolates available at GenBank www.ncbi.nlm.nih.gov/genbank/) and in leBIBI (www.pbil.univ-lyon1.fr/bibi) databases. Antimicrobial susceptibility testing of Nocardia isolates was performed by the broth microdilution method according to CLSI guidelines.
Overall, 342 non-duplicated isolates were obtained. Of them, only 2 (0.6%) were recovered from children < 15 years being both in two immunocompetent patients. Both were primary cutaneous nocardiosis representing 40% of total cutaneous nocardiosis detected. In the first case, a N. farcinica was isolated from a node placed 2 cm away where a 4 months old female had received BCG vaccination. In the second case, a N. brasiliensis was isolated from the elbow of a 9-year old boy who had had an episode of itchy and papulous dermatitis with lesions on the elbows, neck, ankles and torso two months before the onset of the symptoms. Both N. brasiliensis and N. farcinica were susceptible to amoxicillin-clavulanate (MIC = 0.5/9.5 and trimethoprim-sulfamethoxazole) (MIC = 0.5/9.5 µg/ml). N. brasiliensis, showed non-susceptibility to imipenem (MIC = 64µg/ml), ciprofloxacin (MIC >4 µg/ml), linezolid (MIC = 2 µg/ml). N. farcinica was non-susceptible to amoxicillin/ampicillin (MIC = 64µg/ml), clindamycin (MIC >4 µg/ml) and vancomycin (MIC >4 µg/ml).
Discussion:In Gipuzkoa (Basque Country, Spain) pediatric nocardiosis is a rare entity. Two cases, of primary cutaneous nocardiosis were diagnosed in two immunocompetent children, representing <1% of total nocardiosis detected.
Keyword(s): Nocardiosis cutánea, niños menores de 15 años, Nocardia farcinica, Nocardia brasiliensisCOI Other: No conflict of interest