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Abstract
Discussion Forum (0)
Abstract number: 196

Session Type: ePosters

Session Title: ePosters

Authors(s): M.M. Palanca, J. Ruiz, S.J. Guardia, I.M. Portell, L. Martinez, C. Avivar

Authors Affiliations(s): Hospital de Poniente, Spain

Background:

The determination of SARS CoV-2 in cases as deliveries, non-delayed urgent surgeries, transfer to the ICU, etc ... are an emergency in our Hospital that needs to know the result of the RT-PCR as soon as possible. For this reason, we introduce the diagnosis of SARS CoV-2 by rapid PCR, which gives results in one hour, while conventional PCR can take up to approximately 4.5 hours. We make a comparative study of both RT-PCR systems to determine the usefulness of Rapid PCR versus conventional RT-PCR in urgent cases.
 

Methods:


From August 31 to September 24, 2020, a cross-sectional prospective study was carried out to determine the sensitivity of a rapid SARS CoV-2 PCR (Vita-CREDO®) compared to a conventional SARS CoV-2 PCR (Seegene, Werfen® ). A total of 965 samples were analyzed applying the following protocol. The protocol for this study was based on the determination of a rapid PCR and confirmation, using conventional PCR.
 

Results:


47 samples (4.8%) were positive by rapid PCR, so the result was known within one hour to take the necessary measures. 891 samples (92.33%) were negative by rapid PCR and confirmed by conventional PCR. 27 samples (2.79%) obtained a negative result, so they went to conventional PCR by protocol, obtaining a positive result by this other technique. Rapid PCR false negatives determine a specificity of 97.05%. We analyzed the false negatives found by rapid PCR using conventional PCR, finding that the amplification cycles were above 33 CT.
 

Conclusions:


We found the RAPID PCR useful for its use as an urgent PCR in certain urgent cases, using it as a single PCR in deliveries, non-delayed surgery, ICU and hospital transfers, etc. In the event of a positive result, it advances the management of the patient and its diagnosis. In case of negative results in RAPID PCR, it is important to know that it may be due to a patient with high amplification cycles, incubation period, virus elimination, inadequate sample collection ..., therefore, in high suspicion and / or symptomatic patients we would must repeat the study by conventional PCR.
 

Keyword(s): sars cov2, Urgent care, rapid diagnosis

Abstract number: 196

Session Type: ePosters

Session Title: ePosters

Authors(s): M.M. Palanca, J. Ruiz, S.J. Guardia, I.M. Portell, L. Martinez, C. Avivar

Authors Affiliations(s): Hospital de Poniente, Spain

Background:

The determination of SARS CoV-2 in cases as deliveries, non-delayed urgent surgeries, transfer to the ICU, etc ... are an emergency in our Hospital that needs to know the result of the RT-PCR as soon as possible. For this reason, we introduce the diagnosis of SARS CoV-2 by rapid PCR, which gives results in one hour, while conventional PCR can take up to approximately 4.5 hours. We make a comparative study of both RT-PCR systems to determine the usefulness of Rapid PCR versus conventional RT-PCR in urgent cases.
 

Methods:


From August 31 to September 24, 2020, a cross-sectional prospective study was carried out to determine the sensitivity of a rapid SARS CoV-2 PCR (Vita-CREDO®) compared to a conventional SARS CoV-2 PCR (Seegene, Werfen® ). A total of 965 samples were analyzed applying the following protocol. The protocol for this study was based on the determination of a rapid PCR and confirmation, using conventional PCR.
 

Results:


47 samples (4.8%) were positive by rapid PCR, so the result was known within one hour to take the necessary measures. 891 samples (92.33%) were negative by rapid PCR and confirmed by conventional PCR. 27 samples (2.79%) obtained a negative result, so they went to conventional PCR by protocol, obtaining a positive result by this other technique. Rapid PCR false negatives determine a specificity of 97.05%. We analyzed the false negatives found by rapid PCR using conventional PCR, finding that the amplification cycles were above 33 CT.
 

Conclusions:


We found the RAPID PCR useful for its use as an urgent PCR in certain urgent cases, using it as a single PCR in deliveries, non-delayed surgery, ICU and hospital transfers, etc. In the event of a positive result, it advances the management of the patient and its diagnosis. In case of negative results in RAPID PCR, it is important to know that it may be due to a patient with high amplification cycles, incubation period, virus elimination, inadequate sample collection ..., therefore, in high suspicion and / or symptomatic patients we would must repeat the study by conventional PCR.
 

Keyword(s): sars cov2, Urgent care, rapid diagnosis

Comparative study of rapid PCR SARS-CoV-2 versus conventional PCR in urgent patient care
Dra Matilde Maria Palanca
Dra Matilde Maria Palanca
ESCMID eAcademy. Palanca M. 07/09/2021; 327481; 196;
user
Dra Matilde Maria Palanca
Abstract
Discussion Forum (0)
Abstract number: 196

Session Type: ePosters

Session Title: ePosters

Authors(s): M.M. Palanca, J. Ruiz, S.J. Guardia, I.M. Portell, L. Martinez, C. Avivar

Authors Affiliations(s): Hospital de Poniente, Spain

Background:

The determination of SARS CoV-2 in cases as deliveries, non-delayed urgent surgeries, transfer to the ICU, etc ... are an emergency in our Hospital that needs to know the result of the RT-PCR as soon as possible. For this reason, we introduce the diagnosis of SARS CoV-2 by rapid PCR, which gives results in one hour, while conventional PCR can take up to approximately 4.5 hours. We make a comparative study of both RT-PCR systems to determine the usefulness of Rapid PCR versus conventional RT-PCR in urgent cases.
 

Methods:


From August 31 to September 24, 2020, a cross-sectional prospective study was carried out to determine the sensitivity of a rapid SARS CoV-2 PCR (Vita-CREDO®) compared to a conventional SARS CoV-2 PCR (Seegene, Werfen® ). A total of 965 samples were analyzed applying the following protocol. The protocol for this study was based on the determination of a rapid PCR and confirmation, using conventional PCR.
 

Results:


47 samples (4.8%) were positive by rapid PCR, so the result was known within one hour to take the necessary measures. 891 samples (92.33%) were negative by rapid PCR and confirmed by conventional PCR. 27 samples (2.79%) obtained a negative result, so they went to conventional PCR by protocol, obtaining a positive result by this other technique. Rapid PCR false negatives determine a specificity of 97.05%. We analyzed the false negatives found by rapid PCR using conventional PCR, finding that the amplification cycles were above 33 CT.
 

Conclusions:


We found the RAPID PCR useful for its use as an urgent PCR in certain urgent cases, using it as a single PCR in deliveries, non-delayed surgery, ICU and hospital transfers, etc. In the event of a positive result, it advances the management of the patient and its diagnosis. In case of negative results in RAPID PCR, it is important to know that it may be due to a patient with high amplification cycles, incubation period, virus elimination, inadequate sample collection ..., therefore, in high suspicion and / or symptomatic patients we would must repeat the study by conventional PCR.
 

Keyword(s): sars cov2, Urgent care, rapid diagnosis

Abstract number: 196

Session Type: ePosters

Session Title: ePosters

Authors(s): M.M. Palanca, J. Ruiz, S.J. Guardia, I.M. Portell, L. Martinez, C. Avivar

Authors Affiliations(s): Hospital de Poniente, Spain

Background:

The determination of SARS CoV-2 in cases as deliveries, non-delayed urgent surgeries, transfer to the ICU, etc ... are an emergency in our Hospital that needs to know the result of the RT-PCR as soon as possible. For this reason, we introduce the diagnosis of SARS CoV-2 by rapid PCR, which gives results in one hour, while conventional PCR can take up to approximately 4.5 hours. We make a comparative study of both RT-PCR systems to determine the usefulness of Rapid PCR versus conventional RT-PCR in urgent cases.
 

Methods:


From August 31 to September 24, 2020, a cross-sectional prospective study was carried out to determine the sensitivity of a rapid SARS CoV-2 PCR (Vita-CREDO®) compared to a conventional SARS CoV-2 PCR (Seegene, Werfen® ). A total of 965 samples were analyzed applying the following protocol. The protocol for this study was based on the determination of a rapid PCR and confirmation, using conventional PCR.
 

Results:


47 samples (4.8%) were positive by rapid PCR, so the result was known within one hour to take the necessary measures. 891 samples (92.33%) were negative by rapid PCR and confirmed by conventional PCR. 27 samples (2.79%) obtained a negative result, so they went to conventional PCR by protocol, obtaining a positive result by this other technique. Rapid PCR false negatives determine a specificity of 97.05%. We analyzed the false negatives found by rapid PCR using conventional PCR, finding that the amplification cycles were above 33 CT.
 

Conclusions:


We found the RAPID PCR useful for its use as an urgent PCR in certain urgent cases, using it as a single PCR in deliveries, non-delayed surgery, ICU and hospital transfers, etc. In the event of a positive result, it advances the management of the patient and its diagnosis. In case of negative results in RAPID PCR, it is important to know that it may be due to a patient with high amplification cycles, incubation period, virus elimination, inadequate sample collection ..., therefore, in high suspicion and / or symptomatic patients we would must repeat the study by conventional PCR.
 

Keyword(s): sars cov2, Urgent care, rapid diagnosis

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